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Characterisation of stone and pome fruit genotypes by means of molecular techniques
Thesis (MSc) -- Stellenbosch University, 1993.
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| Format: | Thesis |
| Language: | English |
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Stellenbosch : Stellenbosch University
2012
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| _version_ | 1867613994701815809 |
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| access_status_str | Open Access |
| author | Goedhart, Marlene Ann |
| author2 | Retief, A. E. |
| author_browse | Goedhart, Marlene Ann Retief, A. E. |
| author_facet | Retief, A. E. Goedhart, Marlene Ann |
| author_sort | Goedhart, Marlene Ann |
| collection | Thesis |
| dc_rights_str_mv | Stellenbosch University |
| description | Thesis (MSc) -- Stellenbosch University, 1993. |
| format | Thesis |
| id | oai:scholar.sun.ac.za:10019.1/57883 |
| institution | Stellenbosch University (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:44:59.428Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from SUNScholar — Stellenbosch University Repository |
| publishDate | 2012 |
| publishDateRange | 2012 |
| publishDateSort | 2012 |
| publisher | Stellenbosch : Stellenbosch University |
| publisherStr | Stellenbosch : Stellenbosch University |
| record_format | dspace |
| source_str | SUNScholar — Stellenbosch University Repository |
| spelling | oai:scholar.sun.ac.za:10019.1/57883 Characterisation of stone and pome fruit genotypes by means of molecular techniques Goedhart, Marlene Ann Retief, A. E. Mansvelt, E. L. Stellenbosch University. Faculty of AgriSciences. Dept. of Genetics and Institute of Plant Biotechnology. Fruit -- Genetics DNA fingerprinting Fruit -- Identification Fruit -- Varieties -- Identification Pear -- Varieties -- Identification Plum -- Varieties -- Identification Peach -- Varieties -- Identification Dissertations -- Genetics Thesis (MSc) -- Stellenbosch University, 1993. Random amplified polymorphic DNA (RAPD) markers were used to generate DNA fingerprints for 35 deciduous fruit cultivars. RAPD fingerprinting involves the enzymatic amplification of genomic DNA, directed by single primers of arbitrary sequence, using low stringency polymerase chain reaction (PCR) conditions. This low stringency PCR comprised lower than normal primer annealing temperatures, i.e. 37°C for the pears and 32°C for the peaches and plums. The primers used were 10-mers of random sequence, with a G+C content of 50 to 70%. The reaction conditions were optimized to ensure reproducibility. The amplification products were separated on 1.5% agarose gels, stained with ethidium bromide and visualised by UV illumination. The number of bands produced ranged from 3 to 16 with a size range from 0.2 to 3.0 kb. Only the consistent bands were scored for their presence or absence, disregarding differences in intensities. All experiments were repeated at least three times to verify the results. The five pear cultivars that were initially screened with 20 primers illustrated that a high level of genetic variation exists in pears because 40% of the bands detected were polymorphic. This was high when compared to the peaches, where only 3% of the bands detected were polymorphic. A system of sequentially grouping the pear cultivars according to the presence or absence of a polymorphic band was devised and proved to be a useful strategy for differentiating the pear cultivars that display a high level of homology. The level of genetic variation in plums was not determined, but it is expected to be similar to that of the pears because the primers used to type the plums were highly informative. The levels of variability correlate to the origins of these cultivars. Thirteen primers were used to differentiate 35 cultivars. Except for two plum cultivars which generated identical fingerprints, all the cultivars were differentiated. Seventeen pear cultivars were distinguished with three primers, 13 of the 15 plum cultivars with two, and 13 peach cultivars with eight primers. RAPD fingerprinting therefore provides a quick and reliable technique to differentiate pear, plum and peach cultivars. These results are presented in the form of publications, according to the requirements of the journal Theoretical and Applied Genetics. Masters 2012-08-27T11:38:42Z 2012-08-27T11:38:42Z 1993 Thesis http://hdl.handle.net/10019.1/57883 en Stellenbosch University 79 pages : ill. application/pdf Stellenbosch : Stellenbosch University |
| spellingShingle | Fruit -- Genetics DNA fingerprinting Fruit -- Identification Fruit -- Varieties -- Identification Pear -- Varieties -- Identification Plum -- Varieties -- Identification Peach -- Varieties -- Identification Dissertations -- Genetics Goedhart, Marlene Ann Characterisation of stone and pome fruit genotypes by means of molecular techniques |
| title | Characterisation of stone and pome fruit genotypes by means of molecular techniques |
| title_full | Characterisation of stone and pome fruit genotypes by means of molecular techniques |
| title_fullStr | Characterisation of stone and pome fruit genotypes by means of molecular techniques |
| title_full_unstemmed | Characterisation of stone and pome fruit genotypes by means of molecular techniques |
| title_short | Characterisation of stone and pome fruit genotypes by means of molecular techniques |
| title_sort | characterisation of stone and pome fruit genotypes by means of molecular techniques |
| topic | Fruit -- Genetics DNA fingerprinting Fruit -- Identification Fruit -- Varieties -- Identification Pear -- Varieties -- Identification Plum -- Varieties -- Identification Peach -- Varieties -- Identification Dissertations -- Genetics |
| url | http://hdl.handle.net/10019.1/57883 |
| work_keys_str_mv | AT goedhartmarleneann characterisationofstoneandpomefruitgenotypesbymeansofmoleculartechniques |