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Genetic engineering of Saccharomyces cerevisiae for efficient expression and secretion of glucoamylase and a-amylase
Thesis (M. Sc.) -- University of Stellenbosch, 1993.
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| Format: | Thesis |
| Language: | English |
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Stellenbosch : Stellenbosch University
2012
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| _version_ | 1867613926902988800 |
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| access_status_str | Open Access |
| author | D'Aguanno, Vincenzo Silvano |
| author2 | Pretorius, I. S. |
| author_browse | D'Aguanno, Vincenzo Silvano Pretorius, I. S. |
| author_facet | Pretorius, I. S. D'Aguanno, Vincenzo Silvano |
| author_sort | D'Aguanno, Vincenzo Silvano |
| collection | Thesis |
| dc_rights_str_mv | Stellenbosch University |
| description | Thesis (M. Sc.) -- University of Stellenbosch, 1993. |
| format | Thesis |
| id | oai:scholar.sun.ac.za:10019.1/57994 |
| institution | Stellenbosch University (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:43:54.909Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from SUNScholar — Stellenbosch University Repository |
| publishDate | 2012 |
| publishDateRange | 2012 |
| publishDateSort | 2012 |
| publisher | Stellenbosch : Stellenbosch University |
| publisherStr | Stellenbosch : Stellenbosch University |
| record_format | dspace |
| source_str | SUNScholar — Stellenbosch University Repository |
| spelling | oai:scholar.sun.ac.za:10019.1/57994 Genetic engineering of Saccharomyces cerevisiae for efficient expression and secretion of glucoamylase and a-amylase D'Aguanno, Vincenzo Silvano Pretorius, I. S. Stellenbosch University. Faculty of Science. Dept. of Microbiology. Saccharomyces cerevisiae Yeast fungi -- Genetic engineering Amylases Dissertations -- Microbiology Thesis (M. Sc.) -- University of Stellenbosch, 1993. The ever-increasing demand for alternative energy (e.g., fuel extenders) and protein (for food and feed supplements) sources has focused on the potential of amylolytic yeasts for the one-step bioconversion of starchy biomass and industrial waste to alcohol and single-cell protein. The efficiency with which starch is hydrolyzed to fermentable sugars depends on the concentration and types of starch-degrading enzymes secreted by amylolytic yeasts. The enzymatic hydrolysis of starch, consisting of linear (amylose) and branched (amylopectin) glucose polymers, is catalyzed by starch-liquefaction (a-amylase) and saccharification (glucoamylase) enzymes. Some of the most prominent amylolytic yeasts such as Lipomyces kononenkoae, Saccharomycopsis fibuligera and Schwanniomyces occidentalis secrete both a-amylase and glucoamylase that synergistically enhance starch degradation. However, amylolytic (diastatic) strains of Saccharomyces cerevisiae (formerly S. diastaticus), producing only glucoamylase, are preferred to the former species for industrial application because S. cerevisiae has a fast growth rate, a high ethanol tolerance, is an efficient ethanol producer, consists or 48% high-quality protein, and has been associated for centuries with food and beverage production. In an effort to supplement the limited amylolytic activity of S. cerevisiae, our laboratory co-expressed the Bacillus amyloliquefaciens a-amylase-encoding gene (AMY1) and the S. cerevisiae var. diastaticus glucoamylase-encoding gene (STA2) in laboratory and industrial strains of S. cerevisiae. However, the STA2 gene from S. cerevisiae var. diastaticus is regulated negatively by a multirious set of gene products in S. cerevisiae. The STA2 gene is repressed in MATalMATa diploid strains of S. cerevisiae and it was previously poturated that a regulatory sequence within the STA2 promoter was responsible for heterozygous diploid repression. Masters 2012-08-27T11:38:45Z 2012-08-27T11:38:45Z 1993 Thesis http://hdl.handle.net/10019.1/57994 en Stellenbosch University 127 pages : ill. application/pdf Stellenbosch : Stellenbosch University |
| spellingShingle | Saccharomyces cerevisiae Yeast fungi -- Genetic engineering Amylases Dissertations -- Microbiology D'Aguanno, Vincenzo Silvano Genetic engineering of Saccharomyces cerevisiae for efficient expression and secretion of glucoamylase and a-amylase |
| title | Genetic engineering of Saccharomyces cerevisiae for efficient expression and secretion of glucoamylase and a-amylase |
| title_full | Genetic engineering of Saccharomyces cerevisiae for efficient expression and secretion of glucoamylase and a-amylase |
| title_fullStr | Genetic engineering of Saccharomyces cerevisiae for efficient expression and secretion of glucoamylase and a-amylase |
| title_full_unstemmed | Genetic engineering of Saccharomyces cerevisiae for efficient expression and secretion of glucoamylase and a-amylase |
| title_short | Genetic engineering of Saccharomyces cerevisiae for efficient expression and secretion of glucoamylase and a-amylase |
| title_sort | genetic engineering of saccharomyces cerevisiae for efficient expression and secretion of glucoamylase and a amylase |
| topic | Saccharomyces cerevisiae Yeast fungi -- Genetic engineering Amylases Dissertations -- Microbiology |
| url | http://hdl.handle.net/10019.1/57994 |
| work_keys_str_mv | AT daguannovincenzosilvano geneticengineeringofsaccharomycescerevisiaeforefficientexpressionandsecretionofglucoamylaseandaamylase |