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A comparative study of the reactivity of anti-HIV sera with native, synthetic and recombinant antigens derived from the envelope gene of HIV-1
Thesis (M. Sc.(Med.)) -- University of Stellenbosch, 1994.
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| Format: | Thesis |
| Language: | English |
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Stellenbosch : Stellenbosch University
2012
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| _version_ | 1867613873445535744 |
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| access_status_str | Open Access |
| author | Eekhout, Tonia |
| author2 | Becker, M. L. B. |
| author_browse | Becker, M. L. B. Eekhout, Tonia |
| author_facet | Becker, M. L. B. Eekhout, Tonia |
| author_sort | Eekhout, Tonia |
| collection | Thesis |
| dc_rights_str_mv | Stellenbosch University |
| description | Thesis (M. Sc.(Med.)) -- University of Stellenbosch, 1994. |
| format | Thesis |
| id | oai:scholar.sun.ac.za:10019.1/58232 |
| institution | Stellenbosch University (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:43:03.527Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from SUNScholar — Stellenbosch University Repository |
| publishDate | 2012 |
| publishDateRange | 2012 |
| publishDateSort | 2012 |
| publisher | Stellenbosch : Stellenbosch University |
| publisherStr | Stellenbosch : Stellenbosch University |
| record_format | dspace |
| source_str | SUNScholar — Stellenbosch University Repository |
| spelling | oai:scholar.sun.ac.za:10019.1/58232 A comparative study of the reactivity of anti-HIV sera with native, synthetic and recombinant antigens derived from the envelope gene of HIV-1 Eekhout, Tonia Becker, M. L. B. Stellenbosch University. Faculty of Medicine & Health Sciences. Dept. of Pathology. Division of Medical Virology. HIV (Viruses) Dissertations -- Medicine Thesis (M. Sc.(Med.)) -- University of Stellenbosch, 1994. The envelope gene of HIV-1 is the region of the genome most subject to nucleotide variation, which leads to amino acid substitutions within the major antigenic domains. With phylogenetic tree analysis using the env gene, HIV-1 isolates can be classified into different subtypes which correspond to distinct geographic areas. The degree of diversity between so called "Western" (America and Europe) and "African" (Central and Southern) HIV-1 isolates has important consequences for serological diagnostic tests which utilise antigens based on common reference strains. Therefore it was of interest to investigate individual patient antibody responses to viral proteins from local HIV-1 strains. The reactivity of HIV positive sera to purified viral antigen from cell-culture was assessed by "in-house" Western blotting. In addition, synthetic 12 mer peptides (aa 598-609), representing a part of the major epitope in the transmembrane glycoprotein gp41, were reacted with HIV positive sera. These peptides had substitutions corresponding to selected African and South African strains. The immuno Slotting assays utilising native and synthetic peptides were not conclusive enough to identify a particular trend of reactivity specific to any one patient's antibody profile, especially with regard to the env protein responses. For this reason, recombinant gp41 peptides (aa 567-635) covering the major antigenic domain of the transmembrane protein, were produced by bacterial expression in E. coli. The targeted gp41 sequences of two African isolates were modified by enzymatic manipulation for cloning into the pMAL expression vector to produce gp41/maltose-binding protein fusion peptides. The induced fusion peptide (epitope) displayed reactivity to HIV-1 positive serum by Western blotting, highlighting the need for further antibody screening to determine the sensitivity of these recombinant viral epitopes. Purification of gp41 peptides based on the amino acid sequence of selected HIV-1 strains and their successful use in immunoassays, has the potential to identify a "representative" antigenic sequence of gp41 specific for antibody detection in South Africa. Masters 2012-08-27T11:38:52Z 2012-08-27T11:38:52Z 1994 Thesis http://hdl.handle.net/10019.1/58232 en Stellenbosch University 147 pages application/pdf Stellenbosch : Stellenbosch University |
| spellingShingle | HIV (Viruses) Dissertations -- Medicine Eekhout, Tonia A comparative study of the reactivity of anti-HIV sera with native, synthetic and recombinant antigens derived from the envelope gene of HIV-1 |
| title | A comparative study of the reactivity of anti-HIV sera with native, synthetic and recombinant antigens derived from the envelope gene of HIV-1 |
| title_full | A comparative study of the reactivity of anti-HIV sera with native, synthetic and recombinant antigens derived from the envelope gene of HIV-1 |
| title_fullStr | A comparative study of the reactivity of anti-HIV sera with native, synthetic and recombinant antigens derived from the envelope gene of HIV-1 |
| title_full_unstemmed | A comparative study of the reactivity of anti-HIV sera with native, synthetic and recombinant antigens derived from the envelope gene of HIV-1 |
| title_short | A comparative study of the reactivity of anti-HIV sera with native, synthetic and recombinant antigens derived from the envelope gene of HIV-1 |
| title_sort | comparative study of the reactivity of anti hiv sera with native synthetic and recombinant antigens derived from the envelope gene of hiv 1 |
| topic | HIV (Viruses) Dissertations -- Medicine |
| url | http://hdl.handle.net/10019.1/58232 |
| work_keys_str_mv | AT eekhouttonia acomparativestudyofthereactivityofantihivserawithnativesyntheticandrecombinantantigensderivedfromtheenvelopegeneofhiv1 AT eekhouttonia comparativestudyofthereactivityofantihivserawithnativesyntheticandrecombinantantigensderivedfromtheenvelopegeneofhiv1 |