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The regeneration of wheat plants from various explant sources via callus tissue
Thesis (M. Sc.) -- University of Stellenbosch, 1994.
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| Format: | Thesis |
| Language: | en_ZA |
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Stellenbosch : Stellenbosch University
2012
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| _version_ | 1867614135394500608 |
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| access_status_str | Open Access |
| author | Conrad, Ferozah |
| author2 | Watts, J. E. |
| author_browse | Conrad, Ferozah Watts, J. E. |
| author_facet | Watts, J. E. Conrad, Ferozah |
| author_sort | Conrad, Ferozah |
| collection | Thesis |
| dc_rights_str_mv | Stellenbosch University |
| description | Thesis (M. Sc.) -- University of Stellenbosch, 1994. |
| format | Thesis |
| id | oai:scholar.sun.ac.za:10019.1/58605 |
| institution | Stellenbosch University (South Africa) |
| language | en_ZA |
| last_indexed | 2026-06-10T12:47:13.687Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from SUNScholar — Stellenbosch University Repository |
| publishDate | 2012 |
| publishDateRange | 2012 |
| publishDateSort | 2012 |
| publisher | Stellenbosch : Stellenbosch University |
| publisherStr | Stellenbosch : Stellenbosch University |
| record_format | dspace |
| source_str | SUNScholar — Stellenbosch University Repository |
| spelling | oai:scholar.sun.ac.za:10019.1/58605 The regeneration of wheat plants from various explant sources via callus tissue Conrad, Ferozah Watts, J. E. Stellenbosch University. Faculty of Science. Dept. of Botany and Zoology. Regeneration (Botany) Callus (Botany) Wheat -- Physiology Plant tissue culture Dissertations -- Botany Thesis (M. Sc.) -- University of Stellenbosch, 1994. The purpose of this study was the regeneration of wheat plants from callus tissue. The initiation of callus tissue was attempted from root apex, coleoptile, embryo and leaf explants. Callus tissue was successfully induced from root apices and embryos but leaves and coleoptiles proved to be unsuitable as explants. Callusing was, however, exhibited on the leaf-base segments. Different methods of regeneration as described in the literature were carries out with callus tissue induced from the explants. The methods of Papenfuss and Carman (1987), Hay (1989) and Chin and Scott (1977) were unsuccessful. This may be attributed to the fact that other cultivars of wheat, with different regeneration potentials, were used by these researchers. Successful regeneration was reported in the literature with a method employing coleoptilar nodes as explants on MS medium containing 1 mg.dm-3 IAA, 1 mg.dm-3 KIN and 146 mg.dm-3 GIn. Although, regeneration was sporadic and no single combination of any growth regulators used produced consistent regeneration, MS medium containing 0.1 mg.dm-3 IAA, 1 mg.dm-3 KIN and 146 mg.dm-3 GIn produced regeneration in two seperate experiments. Regeneration was also observed with MS medium containing 1 mg.dm-3 IAA,, 1 mg.dm-3 KIN and lower concentrations of GIn viz., ; 80 mg.dm-3 and 90 mg.dm-3. Masters 2012-08-27T11:39:03Z 2012-08-27T11:39:03Z 1994 Thesis http://hdl.handle.net/10019.1/58605 en_ZA Stellenbosch University 89 pages : ill. application/pdf Stellenbosch : Stellenbosch University |
| spellingShingle | Regeneration (Botany) Callus (Botany) Wheat -- Physiology Plant tissue culture Dissertations -- Botany Conrad, Ferozah The regeneration of wheat plants from various explant sources via callus tissue |
| title | The regeneration of wheat plants from various explant sources via callus tissue |
| title_full | The regeneration of wheat plants from various explant sources via callus tissue |
| title_fullStr | The regeneration of wheat plants from various explant sources via callus tissue |
| title_full_unstemmed | The regeneration of wheat plants from various explant sources via callus tissue |
| title_short | The regeneration of wheat plants from various explant sources via callus tissue |
| title_sort | regeneration of wheat plants from various explant sources via callus tissue |
| topic | Regeneration (Botany) Callus (Botany) Wheat -- Physiology Plant tissue culture Dissertations -- Botany |
| url | http://hdl.handle.net/10019.1/58605 |
| work_keys_str_mv | AT conradferozah theregenerationofwheatplantsfromvariousexplantsourcesviacallustissue AT conradferozah regenerationofwheatplantsfromvariousexplantsourcesviacallustissue |