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An investigation into the feasibility of culturing rat embryos in sheep serum for the detection of teratogenic substances in ewes
Thesis (M. Sc. Agric.) -- University of Stellenbosch, 1990.
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| Other Authors: | |
| Format: | Thesis |
| Language: | en_ZA |
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Stellenbosch : Stellenbosch University
2012
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| _version_ | 1867613809774952448 |
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| access_status_str | Open Access |
| author | Burgman, Josepha Maria |
| author2 | Morgenthal, J. C. |
| author_browse | Burgman, Josepha Maria Morgenthal, J. C. |
| author_facet | Morgenthal, J. C. Burgman, Josepha Maria |
| author_sort | Burgman, Josepha Maria |
| collection | Thesis |
| dc_rights_str_mv | Stellenbosch University |
| description | Thesis (M. Sc. Agric.) -- University of Stellenbosch, 1990. |
| format | Thesis |
| id | oai:scholar.sun.ac.za:10019.1/67186 |
| institution | Stellenbosch University (South Africa) |
| language | en_ZA |
| last_indexed | 2026-06-10T12:42:03.173Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from SUNScholar — Stellenbosch University Repository |
| publishDate | 2012 |
| publishDateRange | 2012 |
| publishDateSort | 2012 |
| publisher | Stellenbosch : Stellenbosch University |
| publisherStr | Stellenbosch : Stellenbosch University |
| record_format | dspace |
| source_str | SUNScholar — Stellenbosch University Repository |
| spelling | oai:scholar.sun.ac.za:10019.1/67186 An investigation into the feasibility of culturing rat embryos in sheep serum for the detection of teratogenic substances in ewes Burgman, Josepha Maria Morgenthal, J. C. Barry, D. M. Stellenbosch University. Faculty of AgriSciences. Dept. of Animal Science. Embryology -- Cultures and culture media Sheep -- Abnormalities Teratogenic agents Dissertations -- Physiology (Human and animal) Thesis (M. Sc. Agric.) -- University of Stellenbosch, 1990. An in vitro rot embryo culture technique, frequently used to detect the presence of teratogens or nutritional defiencies in relation to reproductive disorders in sera of different species, was studied. The ability of sheep serum alone, as well as sheep serum mixed with rat serum (80% sheep:20% rat serum), to support acceptable growth and development in rat embryos cultured for 48h on media based on this serum, with rat serum as a control, was investigated. According to the medium parameters measured, differences between the three media types were noted. The rat medium had a lower pH (P<0,01), lower bicarbonate levels (P<0,01 ), less glucose {P<0,01), lower total protein (P<0,01) and a higher osmolarity (P<0,01) than the other two media types throughout the culture period. The mixed and sheep medium did not differ from each other initially in pH, bicarbonate levels and glucose. At 48h the mixed medium treated with embryos had lower pH (P<0,01 ) and bicarbonate levels (P<0,01) than the sheep medium. At 24 hand 48h the treated mixed medium had less glucose (P<0,01) than the sheep medium. The mixed medium had a higher osmolarity (P<0,01) and lower total protein (P .0,01) than the sheep medium. Over time an increase in osmolarity (P<O,01) and total protein (P<0,01) was noted in all three medium types. A drop in pH, glucose and bicarbonate levels was noted in all three medium types over time. Masters 2012-08-27T12:09:44Z 2012-08-27T12:09:44Z 1990 Thesis http://hdl.handle.net/10019.1/67186 en_ZA Stellenbosch University 139 pages : ill. application/pdf Stellenbosch : Stellenbosch University |
| spellingShingle | Embryology -- Cultures and culture media Sheep -- Abnormalities Teratogenic agents Dissertations -- Physiology (Human and animal) Burgman, Josepha Maria An investigation into the feasibility of culturing rat embryos in sheep serum for the detection of teratogenic substances in ewes |
| title | An investigation into the feasibility of culturing rat embryos in sheep serum for the detection of teratogenic substances in ewes |
| title_full | An investigation into the feasibility of culturing rat embryos in sheep serum for the detection of teratogenic substances in ewes |
| title_fullStr | An investigation into the feasibility of culturing rat embryos in sheep serum for the detection of teratogenic substances in ewes |
| title_full_unstemmed | An investigation into the feasibility of culturing rat embryos in sheep serum for the detection of teratogenic substances in ewes |
| title_short | An investigation into the feasibility of culturing rat embryos in sheep serum for the detection of teratogenic substances in ewes |
| title_sort | investigation into the feasibility of culturing rat embryos in sheep serum for the detection of teratogenic substances in ewes |
| topic | Embryology -- Cultures and culture media Sheep -- Abnormalities Teratogenic agents Dissertations -- Physiology (Human and animal) |
| url | http://hdl.handle.net/10019.1/67186 |
| work_keys_str_mv | AT burgmanjosephamaria aninvestigationintothefeasibilityofculturingratembryosinsheepserumforthedetectionofteratogenicsubstancesinewes AT burgmanjosephamaria investigationintothefeasibilityofculturingratembryosinsheepserumforthedetectionofteratogenicsubstancesinewes |