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Electrophoretic analysis of the Cordylus cordylus species group (Sauria: Cordylidae) in the southwestern Cape Province, South Africa

Thesis (M. Sc.) -- University of Stellenbosch, 1991.

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Main Author: Brody, Larisa Susanna
Other Authors: Mouton, P. le F. N.
Format: Thesis
Language:en_ZA
Published: Stellenbosch : Stellenbosch University 2012
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access_status_str Open Access
author Brody, Larisa Susanna
author2 Mouton, P. le F. N.
author_browse Brody, Larisa Susanna
Mouton, P. le F. N.
author_facet Mouton, P. le F. N.
Brody, Larisa Susanna
author_sort Brody, Larisa Susanna
collection Thesis
dc_rights_str_mv Stellenbosch University
description Thesis (M. Sc.) -- University of Stellenbosch, 1991.
format Thesis
id oai:scholar.sun.ac.za:10019.1/69249
institution Stellenbosch University (South Africa)
language en_ZA
last_indexed 2026-06-10T12:43:27.297Z
license_str Other — see source repository
provenance_str_mv Harvested via OAI-PMH from SUNScholar — Stellenbosch University Repository
publishDate 2012
publishDateRange 2012
publishDateSort 2012
publisher Stellenbosch : Stellenbosch University
publisherStr Stellenbosch : Stellenbosch University
record_format dspace
source_str SUNScholar — Stellenbosch University Repository
spelling oai:scholar.sun.ac.za:10019.1/69249 Electrophoretic analysis of the Cordylus cordylus species group (Sauria: Cordylidae) in the southwestern Cape Province, South Africa Brody, Larisa Susanna Mouton, P. le F. N. Grant, W. S. Stellenbosch University. Faculty of Science. Dept. of Botany and Zoology. Cordylidae -- Evolution Cordylidae -- Classification Electrophoresis Dissertations -- Zoology Thesis (M. Sc.) -- University of Stellenbosch, 1991. THe study was divided into two main sections. In the first section the tissue-specific expression of 30 enzymes encoded by 49 presumptive loci in Cordylus oelofseni was examined with starch-gel electrophoresis. The aimwas to search for isozymes that could be used in a study to test a proposed hypothesis on the evaluation and biogeography of the species belonging to the Cordylus cordylus species group in the southwestern Cape. The tissue-specific gene expression in C. oelofseni is similar to that in other lizards. One unexpected locus was detected. It was in the Glucose dehydrogenase system, where a presumably new locus expressed only in the femoral glands was observed. Silencing of one locus may have occurred in the Aconitase and Superoxide dismutase enzyme systems. The most valuable tissues to use in an allele frequency study are liver, muscle, duodenum and heart. In the second section of this project, allozyme frequencies were used to test a proposed hypothesis on the southwestern Cape, by determining the biochemical genetic relationships among taxa in this group. The population structures of the three species in quesetion were also analysed. Low levels of heterozygosity were found in C. oelofseni and C.niger, and intermediate levels in C. cordylus. These levels correlate with the geographical ranges of the species. Genetic distances were calculated among species and approximate times of divergence were estimated. These divergence tiems did not agree with the times suggested by other authors. Both phenetic and cladistic approaches were used to assess relationships among the three Cordylus species under consideration. The results suggest that C.niger is monophyletic but that C. oelofseni is polyphyletic, the Landdroskop population being closer to C.cordylus than to the remaining C.oelofseni populations. It has been demonstrated that avergae genetic distances would not be a reliable aid in decisions regarding the taxanomic status of C. oelofseni. Cordylis niger was shown not to be the sister taxon of C.peersi. Proposed relationships within the Cordylus cordylus species group, as well as monophyly of this group, remain unconfirmed as conflicting results were obtained. Masters 2012-08-27T12:26:59Z 2012-08-27T12:26:59Z 1991 Thesis http://hdl.handle.net/10019.1/69249 en_ZA Stellenbosch University 61 pages : ill. application/pdf Stellenbosch : Stellenbosch University
spellingShingle Cordylidae -- Evolution
Cordylidae -- Classification
Electrophoresis
Dissertations -- Zoology
Brody, Larisa Susanna
Electrophoretic analysis of the Cordylus cordylus species group (Sauria: Cordylidae) in the southwestern Cape Province, South Africa
title Electrophoretic analysis of the Cordylus cordylus species group (Sauria: Cordylidae) in the southwestern Cape Province, South Africa
title_full Electrophoretic analysis of the Cordylus cordylus species group (Sauria: Cordylidae) in the southwestern Cape Province, South Africa
title_fullStr Electrophoretic analysis of the Cordylus cordylus species group (Sauria: Cordylidae) in the southwestern Cape Province, South Africa
title_full_unstemmed Electrophoretic analysis of the Cordylus cordylus species group (Sauria: Cordylidae) in the southwestern Cape Province, South Africa
title_short Electrophoretic analysis of the Cordylus cordylus species group (Sauria: Cordylidae) in the southwestern Cape Province, South Africa
title_sort electrophoretic analysis of the cordylus cordylus species group sauria cordylidae in the southwestern cape province south africa
topic Cordylidae -- Evolution
Cordylidae -- Classification
Electrophoresis
Dissertations -- Zoology
url http://hdl.handle.net/10019.1/69249
work_keys_str_mv AT brodylarisasusanna electrophoreticanalysisofthecordyluscordylusspeciesgroupsauriacordylidaeinthesouthwesterncapeprovincesouthafrica