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Seasonal fluctuations in cytokinin levels in Leucadendron rubrum
Thesis (M. Sc.) -- University of Stellenbosch, 1992.
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| Format: | Thesis |
| Language: | English |
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Stellenbosch : Stellenbosch University
2012
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| _version_ | 1867613907982483456 |
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| access_status_str | Open Access |
| author | De Kock, Marianna |
| author2 | Bellstedt, D. U. |
| author_browse | Bellstedt, D. U. De Kock, Marianna |
| author_facet | Bellstedt, D. U. De Kock, Marianna |
| author_sort | De Kock, Marianna |
| collection | Thesis |
| dc_rights_str_mv | Stellenbosch University |
| description | Thesis (M. Sc.) -- University of Stellenbosch, 1992. |
| format | Thesis |
| id | oai:scholar.sun.ac.za:10019.1/69761 |
| institution | Stellenbosch University (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:43:36.943Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from SUNScholar — Stellenbosch University Repository |
| publishDate | 2012 |
| publishDateRange | 2012 |
| publishDateSort | 2012 |
| publisher | Stellenbosch : Stellenbosch University |
| publisherStr | Stellenbosch : Stellenbosch University |
| record_format | dspace |
| source_str | SUNScholar — Stellenbosch University Repository |
| spelling | oai:scholar.sun.ac.za:10019.1/69761 Seasonal fluctuations in cytokinin levels in Leucadendron rubrum De Kock, Marianna Bellstedt, D. U. Theron, K. I. Swart, P. Stellenbosch University. Faculty of Science. Dept. of Biochemistry. Proteaceae -- Cytology Proteaceae -- Growth Dissertations -- Biochemistry Thesis (M. Sc.) -- University of Stellenbosch, 1992. During this study, plants of the dioecious Leucadendron rubrum were collected on the site of the Weikamp weather-station in the Jonkershoek State Forest, Stellenbosch, from July 1990 to September 1991. HPLC was employed to purify the xylem sap that was extracted from these plants and to separate the cytokinins prior to quantitation by ELISA. The cytokinin levels in the purified plant samples were determined using rabbit antiserum-based ELISA's for the determination of the levels of the cytokinins DHZR and IPA, and a trans-ZR specific monoclonal antibody-based ELISA for the determination of the levels of the cytokinin ZR. It was observed that the levels of the cytokinins DHZR and ZR correlated with the seasonal growth patterns observed in male and female plants. The levels of the cytokinin IPA, in male and female plants of L. rubrum respectively, correlated specifically with the different seasonal growth patterns observed. Peaks in the levels of all three cytokinins, i.e. ZR, DHZR and IPA, occurred during the study period. The peaks corresponded with the phenological development of L. rubrum, specifically with vegetative bud break and inflorescence differentiation. It was concluded that the cytokinin levels appeared to be synchronised with the growth and development of the plants. An attempt was also made to correlate cytokinin levels with the extreme differences in morphological characters exhibited by male and female plants, and to determine to what extent these phytohormones influenced sex expression in L. rubrum. In order to possibly explain both these phenomena, further studies will have to be undertaken on hormone receptors and the levels of all phytohormones will have to be determined in the annual growth cycle of male and female L . rubrum plants. Masters 2012-08-27T12:27:15Z 2012-08-27T12:27:15Z 1992 Thesis http://hdl.handle.net/10019.1/69761 en Stellenbosch University 134 pages : ill. application/pdf Stellenbosch : Stellenbosch University |
| spellingShingle | Proteaceae -- Cytology Proteaceae -- Growth Dissertations -- Biochemistry De Kock, Marianna Seasonal fluctuations in cytokinin levels in Leucadendron rubrum |
| title | Seasonal fluctuations in cytokinin levels in Leucadendron rubrum |
| title_full | Seasonal fluctuations in cytokinin levels in Leucadendron rubrum |
| title_fullStr | Seasonal fluctuations in cytokinin levels in Leucadendron rubrum |
| title_full_unstemmed | Seasonal fluctuations in cytokinin levels in Leucadendron rubrum |
| title_short | Seasonal fluctuations in cytokinin levels in Leucadendron rubrum |
| title_sort | seasonal fluctuations in cytokinin levels in leucadendron rubrum |
| topic | Proteaceae -- Cytology Proteaceae -- Growth Dissertations -- Biochemistry |
| url | http://hdl.handle.net/10019.1/69761 |
| work_keys_str_mv | AT dekockmarianna seasonalfluctuationsincytokininlevelsinleucadendronrubrum |