Full Text Available
Note: Clicking the button above will open the full text document at the original institutional repository in a new window.
Characterization of isoforms of the serum amyloid A protein (SAA) superfamily
Thesis (Ph.D.(Med.)) -- University of Stellenbosch, 1993.
Saved in:
| Main Author: | |
|---|---|
| Other Authors: | |
| Format: | Thesis |
| Language: | English |
| Published: |
Stellenbosch : Stellenbosch University
2012
|
| Subjects: | |
| Tags: |
No Tags, Be the first to tag this record!
|
| _version_ | 1867613954372534272 |
|---|---|
| access_status_str | Open Access |
| author | De Beer, Maria Cecilia |
| author2 | Taljaard, J. J. F. |
| author_browse | De Beer, Maria Cecilia Taljaard, J. J. F. |
| author_facet | Taljaard, J. J. F. De Beer, Maria Cecilia |
| author_sort | De Beer, Maria Cecilia |
| collection | Thesis |
| dc_rights_str_mv | Stellenbosch University |
| description | Thesis (Ph.D.(Med.)) -- University of Stellenbosch, 1993. |
| format | Thesis |
| id | oai:scholar.sun.ac.za:10019.1/70131 |
| institution | Stellenbosch University (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:44:21.236Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from SUNScholar — Stellenbosch University Repository |
| publishDate | 2012 |
| publishDateRange | 2012 |
| publishDateSort | 2012 |
| publisher | Stellenbosch : Stellenbosch University |
| publisherStr | Stellenbosch : Stellenbosch University |
| record_format | dspace |
| source_str | SUNScholar — Stellenbosch University Repository |
| spelling | oai:scholar.sun.ac.za:10019.1/70131 Characterization of isoforms of the serum amyloid A protein (SAA) superfamily De Beer, Maria Cecilia Taljaard, J. J. F. Stellenbosch University. Faculty of Medicine and Health Sciences. Dept. of Pathology. Division of Chemical Pathology. Proteins -- Research Lipoproteins Dissertations -- Medicine Thesis (Ph.D.(Med.)) -- University of Stellenbosch, 1993. Serum amyloid A protein (SAA) was discovered and characterized as a major acute phase protein produced in the liver upon inflammatory cytokine stimulation. As an apolipoprotein of high density lipoprorein (HDL) it displaces apo-A1 and remodels the HDL particle during inflammation. In this thesis new members of the SAA superfamily are identified and characterized in man and mouse. This was achieved by correlating analytical protein data with the known disposition of the genes of these molecules. The SAA superfamily is shown to consist as 2 distinct subfamilies. The one subfamily encompasses the classical acute phase SAA's which are referred to as A-SAAs. The other subfamily comprises the major form of SAA on normal HDL. It is constitutively expressed and is present as minor apolipoprotein components of normal HDL. These molecules, C-SAA in man and SAA5 in the mouse, is postulated to be involved in the normal metabolism of HDL. Further characterization of the inflammatory A-SAA's was pursued in the mouse to identify an amyloid-resistant mouse strain. To this end it is shown that in the CE/J mouse which is amyloid-resistant, only a single inflammatory A-SAA molecule exists which shares characteristics of both SAA1 and SAA2. The cloning of the gene of this molecule is described and it offers the potential for site-directed mutagenesis to identify a structural motif that would allow for selective deposition of certain A-SAA's into amyloid. The studies presented here promote the understanding of the SAA superfamily which, being so well-conserved through evolution, is likely to be of biological importance. Doctoral 2012-08-27T14:03:43Z 2012-08-27T14:03:43Z 1900 Thesis http://hdl.handle.net/10019.1/70131 en Stellenbosch University 136 pages application/pdf Stellenbosch : Stellenbosch University |
| spellingShingle | Proteins -- Research Lipoproteins Dissertations -- Medicine De Beer, Maria Cecilia Characterization of isoforms of the serum amyloid A protein (SAA) superfamily |
| title | Characterization of isoforms of the serum amyloid A protein (SAA) superfamily |
| title_full | Characterization of isoforms of the serum amyloid A protein (SAA) superfamily |
| title_fullStr | Characterization of isoforms of the serum amyloid A protein (SAA) superfamily |
| title_full_unstemmed | Characterization of isoforms of the serum amyloid A protein (SAA) superfamily |
| title_short | Characterization of isoforms of the serum amyloid A protein (SAA) superfamily |
| title_sort | characterization of isoforms of the serum amyloid a protein saa superfamily |
| topic | Proteins -- Research Lipoproteins Dissertations -- Medicine |
| url | http://hdl.handle.net/10019.1/70131 |
| work_keys_str_mv | AT debeermariacecilia characterizationofisoformsoftheserumamyloidaproteinsaasuperfamily |