Full Text Available
Note: Clicking the button above will open the full text document at the original institutional repository in a new window.
The optimization of the extraction and purification of horseradish peroxidase from horseradish roots
Thesis (MSc)--Stellenbosch University, 2012.
Saved in:
| Main Author: | |
|---|---|
| Other Authors: | |
| Format: | Thesis |
| Language: | en_ZA |
| Published: |
Stellenbosch : Stellenbosch University
2012
|
| Subjects: | |
| Tags: |
No Tags, Be the first to tag this record!
|
| _version_ | 1867614088129937408 |
|---|---|
| access_status_str | Open Access |
| author | Barnard, Almero |
| author2 | Swart, Pieter |
| author_browse | Barnard, Almero Swart, Pieter |
| author_facet | Swart, Pieter Barnard, Almero |
| author_sort | Barnard, Almero |
| collection | Thesis |
| dc_rights_str_mv | Stellenbosch University |
| description | Thesis (MSc)--Stellenbosch University, 2012. |
| format | Thesis |
| id | oai:scholar.sun.ac.za:10019.1/71784 |
| institution | Stellenbosch University (South Africa) |
| language | en_ZA |
| last_indexed | 2026-06-10T12:46:28.519Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from SUNScholar — Stellenbosch University Repository |
| publishDate | 2012 |
| publishDateRange | 2012 |
| publishDateSort | 2012 |
| publisher | Stellenbosch : Stellenbosch University |
| publisherStr | Stellenbosch : Stellenbosch University |
| record_format | dspace |
| source_str | SUNScholar — Stellenbosch University Repository |
| spelling | oai:scholar.sun.ac.za:10019.1/71784 The optimization of the extraction and purification of horseradish peroxidase from horseradish roots Barnard, Almero Swart, Pieter Graz, Michael Stellenbosch University. Faculty of Science. Dept. of Biochemistry. Horseradish peroxidase Enzymes -- Purification Dissertations -- Biochemistry Theses -- Biochemistry Biochemistry Thesis (MSc)--Stellenbosch University, 2012. ENGLISH ABSTRACT: This study describes: a) the optimization of the current industrial-scale extraction and purification of Horseradish peroxidase from horseradish at BBI Enzymes, focussing on: a. Raw material quality, b. Extraction, c. Ultra-filtration, d. Salt fractionation, e. Diafiltration, f. Ion Exchange Chromatography, b) developing an new in-process microtitre plate calorimetric assay, c) characterization of main groups of HRP relevant to BBI Enzymes by SDS-PAGE- and HPLC analysis. AFRIKAANSE OPSOMMING: Hierdie studie beskryf: a) die optimisering van die huidige industriële-skaal ekstraksie en suiwering van peperwortelperoksidase vanuit peperwortel by BBI Enzymes, deur te focus op: a. Rou material kwaliteit, b. Ekstraksie, c. Ultra-filtrasie, d. Sout fraksionering, e. Diafiltrasie, f. Ioon-uitruilchromatografie b) Ontwikkeling van ‘n nuwe in-proses mikro-titer gebaseerde kalorimetriese toetsmetode c) die karakterisering van die hoof groepe peperwortel-peroksidase belangrik vir BBI Enzymes. 2012-11-30T06:41:34Z 2012-12-12T08:11:23Z 2012-11-30T06:41:34Z 2012-12-12T08:11:23Z 2012-12 Thesis http://hdl.handle.net/10019.1/71784 en_ZA Stellenbosch University 132 p. : ill. application/pdf Stellenbosch : Stellenbosch University |
| spellingShingle | Horseradish peroxidase Enzymes -- Purification Dissertations -- Biochemistry Theses -- Biochemistry Biochemistry Barnard, Almero The optimization of the extraction and purification of horseradish peroxidase from horseradish roots |
| title | The optimization of the extraction and purification of horseradish peroxidase from horseradish roots |
| title_full | The optimization of the extraction and purification of horseradish peroxidase from horseradish roots |
| title_fullStr | The optimization of the extraction and purification of horseradish peroxidase from horseradish roots |
| title_full_unstemmed | The optimization of the extraction and purification of horseradish peroxidase from horseradish roots |
| title_short | The optimization of the extraction and purification of horseradish peroxidase from horseradish roots |
| title_sort | optimization of the extraction and purification of horseradish peroxidase from horseradish roots |
| topic | Horseradish peroxidase Enzymes -- Purification Dissertations -- Biochemistry Theses -- Biochemistry Biochemistry |
| url | http://hdl.handle.net/10019.1/71784 |
| work_keys_str_mv | AT barnardalmero theoptimizationoftheextractionandpurificationofhorseradishperoxidasefromhorseradishroots AT barnardalmero optimizationoftheextractionandpurificationofhorseradishperoxidasefromhorseradishroots |