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Laboratory optimization of a protease extraction and purification process from bovine pancreas in preparation for industrial scale up
Thesis (MSc)--Stellenbosch University, 2012.
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| Format: | Thesis |
| Language: | en_ZA |
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Stellenbosch : Stellenbosch University
2012
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| _version_ | 1867614031586525184 |
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| access_status_str | Open Access |
| author | De Wet, Tinus Andre |
| author2 | Swart, Pieter |
| author_browse | De Wet, Tinus Andre Swart, Pieter |
| author_facet | Swart, Pieter De Wet, Tinus Andre |
| author_sort | De Wet, Tinus Andre |
| collection | Thesis |
| dc_rights_str_mv | Stellenbosch University |
| description | Thesis (MSc)--Stellenbosch University, 2012. |
| format | Thesis |
| id | oai:scholar.sun.ac.za:10019.1/71790 |
| institution | Stellenbosch University (South Africa) |
| language | en_ZA |
| last_indexed | 2026-06-10T12:45:33.890Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from SUNScholar — Stellenbosch University Repository |
| publishDate | 2012 |
| publishDateRange | 2012 |
| publishDateSort | 2012 |
| publisher | Stellenbosch : Stellenbosch University |
| publisherStr | Stellenbosch : Stellenbosch University |
| record_format | dspace |
| source_str | SUNScholar — Stellenbosch University Repository |
| spelling | oai:scholar.sun.ac.za:10019.1/71790 Laboratory optimization of a protease extraction and purification process from bovine pancreas in preparation for industrial scale up De Wet, Tinus Andre Swart, Pieter Graz, Michael Stellenbosch University. Faculty of Science. Dept. of Biochemistry. Proteins -- Purification Trypsin Proteolytic enzymes Dissertations -- Biochemistry Theses -- Biochemistry Biochemistry Thesis (MSc)--Stellenbosch University, 2012. ENGLISH ABSTRACT: This study describes: a) Characterization of traditional methodologies and testing methods used to purify and quantify trypsin and α-chymotrypsin b) Re-engineering / development of a new method for purifying trypsin and α-chymotrypsin that delivered higher product yields and improved control exercised over the process by investigating: i. Extraction methods ii. Centrifugation iii. Ultrafiltration iv. Chymotrypsinogen and trypsin crystallization v. Column chromatography vi. Investigation into different raw material sources for pancreatic enzyme production c) Development of kinetic and ELISA testing methodologies for in-process QC analysis. AFRIKAANSE OPSOMMING: Hierdie Studie beskryf: a) Karakterisering van die ou prosessering metodes en toets metodes wat gebruik word om Tripsien en Alpha-chimotripsien te suiwer en te kwantifiseer. b) Herontwerp / ontwikkeling van 'n nuwe metode vir die suiwering Tripsien en Chimotripsien wat „n hoër opbrengs lewer en meer kontrole oor die proses uit oefen deur ondersoek in te stel na: i. Ekstraksie- metodes ii. Sentrifugering iii. Ultrafiltrasie iv. Chymotripsienogeen - en tripsien kristallisasie v. Kolom chromatografie vi. Ondersoek na verskillende rou materiaal bronne vir die produksie van pankreas ensieme. c) Die ontwikkeling van kinetiese- en ELISA toets metodes vir die in-proses kwaliteitkontrole. 2012-12-01T16:26:10Z 2012-12-12T08:11:28Z 2012-12-01T16:26:10Z 2012-12-12T08:11:28Z 2012-12 Thesis http://hdl.handle.net/10019.1/71790 en_ZA Stellenbosch University 199 p. : col. ill. application/pdf Stellenbosch : Stellenbosch University |
| spellingShingle | Proteins -- Purification Trypsin Proteolytic enzymes Dissertations -- Biochemistry Theses -- Biochemistry Biochemistry De Wet, Tinus Andre Laboratory optimization of a protease extraction and purification process from bovine pancreas in preparation for industrial scale up |
| title | Laboratory optimization of a protease extraction and purification process from bovine pancreas in preparation for industrial scale up |
| title_full | Laboratory optimization of a protease extraction and purification process from bovine pancreas in preparation for industrial scale up |
| title_fullStr | Laboratory optimization of a protease extraction and purification process from bovine pancreas in preparation for industrial scale up |
| title_full_unstemmed | Laboratory optimization of a protease extraction and purification process from bovine pancreas in preparation for industrial scale up |
| title_short | Laboratory optimization of a protease extraction and purification process from bovine pancreas in preparation for industrial scale up |
| title_sort | laboratory optimization of a protease extraction and purification process from bovine pancreas in preparation for industrial scale up |
| topic | Proteins -- Purification Trypsin Proteolytic enzymes Dissertations -- Biochemistry Theses -- Biochemistry Biochemistry |
| url | http://hdl.handle.net/10019.1/71790 |
| work_keys_str_mv | AT dewettinusandre laboratoryoptimizationofaproteaseextractionandpurificationprocessfrombovinepancreasinpreparationforindustrialscaleup |