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Preparation and surface-functionalization of dye-doped bioconjugated poly(styrene-co-maleimide) nanoparticles
Thesis (MSc)--Stellenbosch University, 2014.
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| Format: | Thesis |
| Language: | en_ZA |
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Stellenbosch : Stellenbosch University
2015
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| _version_ | 1867614010353909760 |
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| access_status_str | Open Access |
| author | Swanepoel, Andri |
| author2 | Klumperman, Bert |
| author_browse | Klumperman, Bert Swanepoel, Andri |
| author_facet | Klumperman, Bert Swanepoel, Andri |
| author_sort | Swanepoel, Andri |
| collection | Thesis |
| dc_rights_str_mv | Stellenbosch University |
| description | Thesis (MSc)--Stellenbosch University, 2014. |
| format | Thesis |
| id | oai:scholar.sun.ac.za:10019.1/95785 |
| institution | Stellenbosch University (South Africa) |
| language | en_ZA |
| last_indexed | 2026-06-10T12:45:13.990Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from SUNScholar — Stellenbosch University Repository |
| publishDate | 2015 |
| publishDateRange | 2015 |
| publishDateSort | 2015 |
| publisher | Stellenbosch : Stellenbosch University |
| publisherStr | Stellenbosch : Stellenbosch University |
| record_format | dspace |
| source_str | SUNScholar — Stellenbosch University Repository |
| spelling | oai:scholar.sun.ac.za:10019.1/95785 Preparation and surface-functionalization of dye-doped bioconjugated poly(styrene-co-maleimide) nanoparticles Swanepoel, Andri Klumperman, Bert Chetty, Avashni Stellenbosch University. Faculty of Science. Department of Chemistry and Polymer Science. Pathogenic microorganisms -- Detection Microbial contamination Nanoparticles Fluorescence Drinking water -- Contamination Thesis (MSc)--Stellenbosch University, 2014. ENGLISH ABSTRACT: Standard methods used for detection of microbial contamination in water and food are based on culturing of samples to increase the number of bacteria present to a detectable level. These tests are however time-consuming (requiring one to two days for results to be generated) and labour intensive. The study described in this dissertation aims to develop dye-doped poly(styrene-co-maleimide) nanoparticles conjugated to antibodies for the rapid (within a few hours) detection of E. coli in water via clustering of the nanoparticles around bacteria to create a concentrated and localised fluorescent signal. Pristine and dye-doped nanoparticles were synthesised according to a one-step (published) method, and surface-functionalised with formaldehyde and three different diamine compounds. The percentage dye added to the particles was also varied in the study. The functionalised nanoparticles were characterised via FTIR, TEM, DSC, and Zetasizer. The emission properties of the dye-loaded NPs were measured with a spectrofluorometer. The functionalised nanoparticles were bioconjugated with avidin and antibodies. The extent of avidin attachment was quantified via the use of a biotin(5-fluorescein) conjugate and via an avidin titration assay. The functionality of the bioconjugated nanoparticles was tested via a sandwich assay and a centrifuge based assay with E. coli cultures. Fluorescence microscopy was also utilised to demonstrate the utility of the particles in creating localised fluorescent signals indicating the presence of E. coli cells. 2015-01-13T11:46:56Z 2015-11-18T03:00:09Z 2014-12 Thesis http://hdl.handle.net/10019.1/95785 en_ZA Stellenbosch University xiii, 130 pages : illustrations (some colour) application/pdf application/pdf Stellenbosch : Stellenbosch University |
| spellingShingle | Pathogenic microorganisms -- Detection Microbial contamination Nanoparticles Fluorescence Drinking water -- Contamination Swanepoel, Andri Preparation and surface-functionalization of dye-doped bioconjugated poly(styrene-co-maleimide) nanoparticles |
| title | Preparation and surface-functionalization of dye-doped bioconjugated poly(styrene-co-maleimide) nanoparticles |
| title_full | Preparation and surface-functionalization of dye-doped bioconjugated poly(styrene-co-maleimide) nanoparticles |
| title_fullStr | Preparation and surface-functionalization of dye-doped bioconjugated poly(styrene-co-maleimide) nanoparticles |
| title_full_unstemmed | Preparation and surface-functionalization of dye-doped bioconjugated poly(styrene-co-maleimide) nanoparticles |
| title_short | Preparation and surface-functionalization of dye-doped bioconjugated poly(styrene-co-maleimide) nanoparticles |
| title_sort | preparation and surface functionalization of dye doped bioconjugated poly styrene co maleimide nanoparticles |
| topic | Pathogenic microorganisms -- Detection Microbial contamination Nanoparticles Fluorescence Drinking water -- Contamination |
| url | http://hdl.handle.net/10019.1/95785 |
| work_keys_str_mv | AT swanepoelandri preparationandsurfacefunctionalizationofdyedopedbioconjugatedpolystyrenecomaleimidenanoparticles |