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The inhibitory effect of rooibos on cytochromes P450 and downstream in vitro modulation of steroid hormones
Thesis (MSc)--Stellenbosch University, 2015.
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| Format: | Thesis |
| Language: | en_ZA |
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Stellenbosch : Stellenbosch University
2015
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| _version_ | 1867614114339094528 |
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| access_status_str | Open Access |
| author | Mugari, Mufaro Buhlebenkosi |
| author2 | Swart, Amanda C. |
| author_browse | Mugari, Mufaro Buhlebenkosi Swart, Amanda C. |
| author_facet | Swart, Amanda C. Mugari, Mufaro Buhlebenkosi |
| author_sort | Mugari, Mufaro Buhlebenkosi |
| collection | Thesis |
| dc_rights_str_mv | Stellenbosch University |
| description | Thesis (MSc)--Stellenbosch University, 2015. |
| format | Thesis |
| id | oai:scholar.sun.ac.za:10019.1/96680 |
| institution | Stellenbosch University (South Africa) |
| language | en_ZA |
| last_indexed | 2026-06-10T12:46:53.692Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from SUNScholar — Stellenbosch University Repository |
| publishDate | 2015 |
| publishDateRange | 2015 |
| publishDateSort | 2015 |
| publisher | Stellenbosch : Stellenbosch University |
| publisherStr | Stellenbosch : Stellenbosch University |
| record_format | dspace |
| source_str | SUNScholar — Stellenbosch University Repository |
| spelling | oai:scholar.sun.ac.za:10019.1/96680 The inhibitory effect of rooibos on cytochromes P450 and downstream in vitro modulation of steroid hormones Mugari, Mufaro Buhlebenkosi Swart, Amanda C. Swart, Pieter Stellenbosch University. Faculty of Science. Dept. of Biochemistry. Cytochrome P-450 Steroid hormones Rooibos tea -- Therapeutic use UCTD Thesis (MSc)--Stellenbosch University, 2015. ENGLISH ABSTRACT: This study describes: 1. Substrate binding assays investigating the effects of methanolic extracts of unfermented and fermented Rooibos on the binding of natural substrates to ovine adrenal microsomal and mitochondrial P450 enzymes, demonstrating the interference of substrate binding in the presence of the Rooibos extracts. 2. The effects of selected flavonoids (quercetin, rutin and aspalathin) on the binding of natural substrates to ovine adrenal microsomal and mitochondrial P450 enzymes, demonstrating interference of substrate binding in the presence of the flavonoid compounds. 3. Substrate conversion assays in non-steroidogenic COS-1 cells to investigate the effects of methanolic extracts of unfermented and fermented Rooibos on the activity of key steroidogenic P450 enzymes (CYP17A1, CYP21A2, CYP11B1, and CYP11B2), demonstrating inhibition of the catalytic activity in the presence of Rooibos extracts. 4. The effects of selected flavonoids on the substrate conversion of the aforementioned key steroidogenic enzymes expressed in COS-1 cells. 5. An investigation of the effect of methanolic extracts of unfermented and fermented Rooibos on steroid hormone production in human adrenal H295R cells under basal and stimulated conditions, demonstrating the modulating effects of unfermented and fermented Rooibos extracts. Basal and stimulated steroid hormone production was decreased in the presence of unfermented and fermented Rooibos. AFRIKAANSE OPSOMMING: Hierdie studie beskryf: 1. Die gebruik van substraatbindings-essais om die effek van metanoliese ekstrakte, van gefermenteerde- en ongefermenteerde Rooibos, op die binding van die natuurlike substrate aan skaap adrenale mikrosomale en -mitochondriale P450 ensieme te bepaal. Daar is getoon dat die ekstrakte 'n beduidende inhiberende effek op ensiemsubstraatinteraksie gehad het. 2. Die die inhiberende effek van geselekteerde flavonoïede (kwersetien, rutien and aspalatien) op die binding van die natuurlike substrate aan skaap adrenale mikrosomale en -mitochondriale P450 ensieme. 3. Die gebruik van substraatomsettings-essais in nie-steroïedogeniese COS-1 selle, om die effek van gefermenteerde- en ongefermenteerde Rooibos ekstrakte op die aktiwiteit van die steroïedogeniese P450 ensieme (CYP17A1, CYP21A2, CYP11B1, and CYP11B2) se katalitiese aktiwiteit te bepaal. Daar kon aangetoon word dat die katalitise aktiwiteite van bg. ensieme beduidend beïnvloed word deur die Rooibos ekstrakte. 4. Die gebruik van substraatomsettings-essais in nie-steroïedogeniese COS-1 selle, om die effek van geselekteerde flavonoïede op die aktiwiteit van bogenoemde steroïedogeniese P450 ensieme te bepaal. 5. 'n Ondersoek na die invloed van metanoliese ekstrakte van gefermenteerde- en ongefermenteerde Rooibos op steroïedhormoon biosintese in die menslike adrenale H295R-selmodel. Die ondersoek, onder basale en gestimuleerde toestande, het getoon dat beide Rooibosekstrakte in bogenoemde toestande steroïedhormoon produksie geinhibeer het. 2015-05-20T09:13:45Z 2015-05-20T09:13:45Z 2015-04 Thesis http://hdl.handle.net/10019.1/96680 en_ZA Stellenbosch University xvii, 130 pages : colour illustrations application/pdf Stellenbosch : Stellenbosch University |
| spellingShingle | Cytochrome P-450 Steroid hormones Rooibos tea -- Therapeutic use UCTD Mugari, Mufaro Buhlebenkosi The inhibitory effect of rooibos on cytochromes P450 and downstream in vitro modulation of steroid hormones |
| title | The inhibitory effect of rooibos on cytochromes P450 and downstream in vitro modulation of steroid hormones |
| title_full | The inhibitory effect of rooibos on cytochromes P450 and downstream in vitro modulation of steroid hormones |
| title_fullStr | The inhibitory effect of rooibos on cytochromes P450 and downstream in vitro modulation of steroid hormones |
| title_full_unstemmed | The inhibitory effect of rooibos on cytochromes P450 and downstream in vitro modulation of steroid hormones |
| title_short | The inhibitory effect of rooibos on cytochromes P450 and downstream in vitro modulation of steroid hormones |
| title_sort | inhibitory effect of rooibos on cytochromes p450 and downstream in vitro modulation of steroid hormones |
| topic | Cytochrome P-450 Steroid hormones Rooibos tea -- Therapeutic use UCTD |
| url | http://hdl.handle.net/10019.1/96680 |
| work_keys_str_mv | AT mugarimufarobuhlebenkosi theinhibitoryeffectofrooibosoncytochromesp450anddownstreaminvitromodulationofsteroidhormones AT mugarimufarobuhlebenkosi inhibitoryeffectofrooibosoncytochromesp450anddownstreaminvitromodulationofsteroidhormones |