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Exploring proteomic and microbiome profiling in pigs fed high fibre diets
Thesis (PhD)--Stellenbosch University, 2015.
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| Format: | Thesis |
| Language: | en_ZA |
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Stellenbosch : Stellenbosch University
2015
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| _version_ | 1867613789834182656 |
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| access_status_str | Open Access |
| author | Kanengoni, Arnold Tapera |
| author2 | Dzama, Kennedy |
| author_browse | Dzama, Kennedy Kanengoni, Arnold Tapera |
| author_facet | Dzama, Kennedy Kanengoni, Arnold Tapera |
| author_sort | Kanengoni, Arnold Tapera |
| collection | Thesis |
| dc_rights_str_mv | Stellenbosch University |
| description | Thesis (PhD)--Stellenbosch University, 2015. |
| format | Thesis |
| id | oai:scholar.sun.ac.za:10019.1/96869 |
| institution | Stellenbosch University (South Africa) |
| language | en_ZA |
| last_indexed | 2026-06-10T12:41:43.824Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from SUNScholar — Stellenbosch University Repository |
| publishDate | 2015 |
| publishDateRange | 2015 |
| publishDateSort | 2015 |
| publisher | Stellenbosch : Stellenbosch University |
| publisherStr | Stellenbosch : Stellenbosch University |
| record_format | dspace |
| source_str | SUNScholar — Stellenbosch University Repository |
| spelling | oai:scholar.sun.ac.za:10019.1/96869 Exploring proteomic and microbiome profiling in pigs fed high fibre diets Kanengoni, Arnold Tapera Dzama, Kennedy Ndimba, B. Chimonyo, M. Stellenbosch University. Faculty of Agrisciences. Dept. of Animal Sciences. Proteomic profiling Microbiome profiling Pigs -- Nutrition Pigs -- High fibre diets UCTD Thesis (PhD)--Stellenbosch University, 2015. ENGLISH ABSTRACT: The aim of this study was to explore proteomics and microbiome profiling in pigs fed high fibre diets. In the first phase, maize cobs were ensiled using whey, molasses and exogenous enzymes in the laboratory and effects on ensiling characteristics and fibre levels were evaluated. In the second phase South African Windsnyer-type Indigenous pigs (SAWIP) and Large White x Landrace crosses (LW x LR) were fed diets containing ensiled maize cobs and evaluated on; diet preferences, nutrient digestibility and colonic fermentation; growth performance, carcass traits and blood metabolite profiles; the faecal microbiome; and serum and liver proteomic profiles. Ensiling maize cobs with molasses, whey and exogenous enzymes did not improve fermentation characteristics but exogenous enzymes reduced fibre fractions and energy content of maize cob silages. Dets containing two levels of maize cobs ensiled without any additive; a low (LMC) and high (HMC) maize cob inclusion levels and a control diet which did not have any maize cobs (CON) were formulated. The SAWIP preferred the CON diet more than (P < 0.05) diets with maize cobs while the LW x LR had no feed preferences. There was no correlation between preference and diet digestibility in both breeds. The SAWIP digested nutrients better (P < 0.05) than the LW x LR in the high fibre diets. There were no differences in the diversity of the core composition of gut bacterial communities between the breeds and diets. There were differences in the ratios of Bacteroidia to Clostridia between the SAWIP and LW x LR. Verrucomicrobiae was present in SAWIP and LW x LR on HMC diet and not on the CON diet. There was a breed x diet interaction (P < 0.05) for Oscillospira. Analysis of the microbiome revealed breed differences and no dietary differences. There were differences in serum and liver proteins and in serum metabolite levels. Two specific proteins identified were Guanidinoacetate N-methyltransferase-like isoform 1 associated with creatine biosynthetic and Catalase, which is involved in cholesterol metabolic processes. At the grower stage, the SAWIP consumed more feed per metabolic body weight than the LW x LR while at the finisher stage LW x LR consumed more feed per metabolic body weight (P < 0.05) than the SAWIP. The breed of pig influenced most of the growth performance and carcass parameters more than the diet did. The SAWIP demonstrated an adaptation to high fibre diets by consuming more feed than the LW x LR per metabolic body weight at the grower stage. The inclusion of ensiled maize cobs in diets did not negatively affect selected commercial pork cuts. Analysis of faecal microbiomes revealed differences that may explain the enhanced ability of the SAWIP to digest fibrous diets better than the LW x LR breed.Proteomics can identify biomarkers that evaluate the performance of pigs consuming high fibre diets. A proof of principle to assess serum and liver protein profiles of pigs fed a a high fibre diet using a sodium dodecyl sulphate polyacrylamide gel electrophoresis matrix-assisted laser desorption ionization mass spectrometry (SDS-PAGE /MALDI MS) workflow was established. Key words: ensiling, exogenous enzymes, palatability, fermentation, fibre, metageome, biomarkers. Doctoral 2015-05-20T09:28:02Z 2015-05-20T09:28:02Z 2015-04 Thesis http://hdl.handle.net/10019.1/96869 en_ZA Stellenbosch University xiv, 112 pages : illustrations application/pdf Stellenbosch : Stellenbosch University |
| spellingShingle | Proteomic profiling Microbiome profiling Pigs -- Nutrition Pigs -- High fibre diets UCTD Kanengoni, Arnold Tapera Exploring proteomic and microbiome profiling in pigs fed high fibre diets |
| title | Exploring proteomic and microbiome profiling in pigs fed high fibre diets |
| title_full | Exploring proteomic and microbiome profiling in pigs fed high fibre diets |
| title_fullStr | Exploring proteomic and microbiome profiling in pigs fed high fibre diets |
| title_full_unstemmed | Exploring proteomic and microbiome profiling in pigs fed high fibre diets |
| title_short | Exploring proteomic and microbiome profiling in pigs fed high fibre diets |
| title_sort | exploring proteomic and microbiome profiling in pigs fed high fibre diets |
| topic | Proteomic profiling Microbiome profiling Pigs -- Nutrition Pigs -- High fibre diets UCTD |
| url | http://hdl.handle.net/10019.1/96869 |
| work_keys_str_mv | AT kanengoniarnoldtapera exploringproteomicandmicrobiomeprofilinginpigsfedhighfibrediets |