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Using XhLEA, a group 1 vegetative Late Embryogenesis Abundant protein to aid water deficit tolerance in plants and microbes
Thesis (MSc)--Stellenbosch University, 2015.
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| Format: | Thesis |
| Language: | en_ZA |
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Stellenbosch : Stellenbosch University
2015
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| _version_ | 1867613977338445824 |
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| access_status_str | Open Access |
| author | Denkhaus, Erik |
| author2 | Peters, Shaun W. |
| author_browse | Denkhaus, Erik Peters, Shaun W. |
| author_facet | Peters, Shaun W. Denkhaus, Erik |
| author_sort | Denkhaus, Erik |
| collection | Thesis |
| dc_rights_str_mv | Stellenbosch University |
| description | Thesis (MSc)--Stellenbosch University, 2015. |
| format | Thesis |
| id | oai:scholar.sun.ac.za:10019.1/98113 |
| institution | Stellenbosch University (South Africa) |
| language | en_ZA |
| last_indexed | 2026-06-10T12:44:42.460Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from SUNScholar — Stellenbosch University Repository |
| publishDate | 2015 |
| publishDateRange | 2015 |
| publishDateSort | 2015 |
| publisher | Stellenbosch : Stellenbosch University |
| publisherStr | Stellenbosch : Stellenbosch University |
| record_format | dspace |
| source_str | SUNScholar — Stellenbosch University Repository |
| spelling | oai:scholar.sun.ac.za:10019.1/98113 Using XhLEA, a group 1 vegetative Late Embryogenesis Abundant protein to aid water deficit tolerance in plants and microbes Denkhaus, Erik Peters, Shaun W. Stellenbosch University. Faculty of Agrisciences. Dept. of Genetics. Institute for Plant Biotechnology (IPB). Late Embryogenesis Abundant (LEA) protein Resurrection plant Water deficit High salinity UCTD Thesis (MSc)--Stellenbosch University, 2015. ENGLISH ABSTRACT: Late Embryogenesis Abundant (LEA) genes have been irrefutably linked to the osmotic stress response since their initial discovery in maturing cotton seeds. They have since been reported from a multitude of other organism where their occurrence is often associated with general responses to abiotic stress. Many studies have been conducted using LEA genes in over expression strategies to improve abiotic stress resistance. Of the known classes of LEAs, the group 1 LEAs have been widely reported, in plants, to only occur in seeds during late stages of development. Their expression coincides with the seeds acquisition of desiccation tolerance. In this thesis we present a group 1 LEA isolated from the desiccated vegetative tissues (leaves) of the resurrection plant Xerophyta humilis. Using E.coli and Arabidopsis we attempted to use XhLEA to improve salt and water deficit stress-responses, respectively. To this end we conducted soil-drought trials on two independent transgenic Arabidopsis lines expressing XhLEA under a drought inducible-promoter and monitored their responses as compared to untransformed WT (Col-0 ) controls. Solid substrate E.coli growth assays and liquid media growth curves under both stress and unstressed conditions were conducted. We found no obvious beneficial effect through the expression of XhLEA in either of the organisms. AFRIKAANSE OPSOMMING: Geen opsomming beskikbaar Masters 2015-12-14T07:44:18Z 2015-12-14T07:44:18Z 2015-12 Thesis http://hdl.handle.net/10019.1/98113 en_ZA Stellenbosch University 45 pages : illustrations application/pdf Stellenbosch : Stellenbosch University |
| spellingShingle | Late Embryogenesis Abundant (LEA) protein Resurrection plant Water deficit High salinity UCTD Denkhaus, Erik Using XhLEA, a group 1 vegetative Late Embryogenesis Abundant protein to aid water deficit tolerance in plants and microbes |
| title | Using XhLEA, a group 1 vegetative Late Embryogenesis Abundant protein to aid water deficit tolerance in plants and microbes |
| title_full | Using XhLEA, a group 1 vegetative Late Embryogenesis Abundant protein to aid water deficit tolerance in plants and microbes |
| title_fullStr | Using XhLEA, a group 1 vegetative Late Embryogenesis Abundant protein to aid water deficit tolerance in plants and microbes |
| title_full_unstemmed | Using XhLEA, a group 1 vegetative Late Embryogenesis Abundant protein to aid water deficit tolerance in plants and microbes |
| title_short | Using XhLEA, a group 1 vegetative Late Embryogenesis Abundant protein to aid water deficit tolerance in plants and microbes |
| title_sort | using xhlea a group 1 vegetative late embryogenesis abundant protein to aid water deficit tolerance in plants and microbes |
| topic | Late Embryogenesis Abundant (LEA) protein Resurrection plant Water deficit High salinity UCTD |
| url | http://hdl.handle.net/10019.1/98113 |
| work_keys_str_mv | AT denkhauserik usingxhleaagroup1vegetativelateembryogenesisabundantproteintoaidwaterdeficittoleranceinplantsandmicrobes |