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PCR bias in 16S rRNA genes caused by GC content leads to insufficient detection of some abundant species in amplicon sequencing analyses of thermophilic microbial communities

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Published in:The Journal of General and Applied Microbiology
Format: Online Article RSS Article
Published: 2025
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container_title The Journal of General and Applied Microbiology
description
discipline_display Natural Sciences
discipline_facet Natural Sciences
format Online Article
RSS Article
genre Journal Article
id rss_article:16589
institution FRELIP
journal_source_facet The Journal of General and Applied Microbiology
publishDate 2025
publishDateSort 2025
record_format rss_article
spellingShingle PCR bias in 16S rRNA genes caused by GC content leads to insufficient detection of some abundant species in amplicon sequencing analyses of thermophilic microbial communities
Microbiology
Natural Sciences — Life Sciences
Natural Sciences
sub_discipline_display Natural Sciences — Life Sciences
sub_discipline_facet Natural Sciences — Life Sciences
subject_display Microbiology
Natural Sciences — Life Sciences
Natural Sciences
Microbiology
Natural Sciences — Life Sciences
Natural Sciences
subject_facet Microbiology
Natural Sciences — Life Sciences
Natural Sciences
title PCR bias in 16S rRNA genes caused by GC content leads to insufficient detection of some abundant species in amplicon sequencing analyses of thermophilic microbial communities
title_auth PCR bias in 16S rRNA genes caused by GC content leads to insufficient detection of some abundant species in amplicon sequencing analyses of thermophilic microbial communities
title_full PCR bias in 16S rRNA genes caused by GC content leads to insufficient detection of some abundant species in amplicon sequencing analyses of thermophilic microbial communities
title_fullStr PCR bias in 16S rRNA genes caused by GC content leads to insufficient detection of some abundant species in amplicon sequencing analyses of thermophilic microbial communities
title_full_unstemmed PCR bias in 16S rRNA genes caused by GC content leads to insufficient detection of some abundant species in amplicon sequencing analyses of thermophilic microbial communities
title_short PCR bias in 16S rRNA genes caused by GC content leads to insufficient detection of some abundant species in amplicon sequencing analyses of thermophilic microbial communities
title_sort pcr bias in 16s rrna genes caused by gc content leads to insufficient detection of some abundant species in amplicon sequencing analyses of thermophilic microbial communities
topic Microbiology
Natural Sciences — Life Sciences
Natural Sciences
url https://pubmed.ncbi.nlm.nih.gov/40414713/?utm_source=Chrome&utm_medium=rss&utm_campaign=None&utm_content=12ukGnx_-plPNzlmyrXx8F1eFGQoACErVTYjKOgfuFGEA7Ys2x&fc=None&ff=20260424002924&v=2.19.0.post6+133c1fe