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PCR bias in 16S rRNA genes caused by GC content leads to insufficient detection of some abundant species in amplicon sequencing analyses of thermophilic microbial communities

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Published in:The Journal of General and Applied Microbiology
Format: Online Article RSS Article
Published: 2025
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container_title The Journal of General and Applied Microbiology
description
discipline_display Microbiology
discipline_facet Microbiology
format Online Article
RSS Article
genre Journal Article
id rss_article:62233
institution FRELIP
journal_source_facet The Journal of General and Applied Microbiology
last_indexed 2026-06-20T21:27:21.959Z
publishDate 2025
publishDateSort 2025
record_format rss_article
spellingShingle PCR bias in 16S rRNA genes caused by GC content leads to insufficient detection of some abundant species in amplicon sequencing analyses of thermophilic microbial communities
Microbiology
General
Microbiology
sub_discipline_display General
sub_discipline_facet General
subject_display Microbiology
General
Microbiology
subject_facet Microbiology
General
Microbiology
title PCR bias in 16S rRNA genes caused by GC content leads to insufficient detection of some abundant species in amplicon sequencing analyses of thermophilic microbial communities
title_alt Sesgo de PCR en genes 16S rRNA causado por el contenido de GC conduce a una detección insuficiente de algunas especies abundantes en análisis de secuenciación de amplicones de comunidades microbianas termófilas
Biais de PCR dans les gènes d'ARNr 16S causé par le contenu en GC conduisant à une détection insuffisante de certaines espèces abondantes dans les analyses de séquençage d'amplicons de communautés microbiennes thermophiles
Viés de PCR em genes 16S rRNA causado pelo conteúdo GC leva à detecção insuficiente de algumas espécies abundantes em análises de sequenciamento de amplicons de comunidades microbianas termofílicas
title_auth PCR bias in 16S rRNA genes caused by GC content leads to insufficient detection of some abundant species in amplicon sequencing analyses of thermophilic microbial communities
title_es_txt Sesgo de PCR en genes 16S rRNA causado por el contenido de GC conduce a una detección insuficiente de algunas especies abundantes en análisis de secuenciación de amplicones de comunidades microbianas termófilas
title_fr_txt Biais de PCR dans les gènes d'ARNr 16S causé par le contenu en GC conduisant à une détection insuffisante de certaines espèces abondantes dans les analyses de séquençage d'amplicons de communautés microbiennes thermophiles
title_full PCR bias in 16S rRNA genes caused by GC content leads to insufficient detection of some abundant species in amplicon sequencing analyses of thermophilic microbial communities
title_fullStr PCR bias in 16S rRNA genes caused by GC content leads to insufficient detection of some abundant species in amplicon sequencing analyses of thermophilic microbial communities
title_full_unstemmed PCR bias in 16S rRNA genes caused by GC content leads to insufficient detection of some abundant species in amplicon sequencing analyses of thermophilic microbial communities
title_pt_txt Viés de PCR em genes 16S rRNA causado pelo conteúdo GC leva à detecção insuficiente de algumas espécies abundantes em análises de sequenciamento de amplicons de comunidades microbianas termofílicas
title_short PCR bias in 16S rRNA genes caused by GC content leads to insufficient detection of some abundant species in amplicon sequencing analyses of thermophilic microbial communities
title_sort pcr bias in 16s rrna genes caused by gc content leads to insufficient detection of some abundant species in amplicon sequencing analyses of thermophilic microbial communities
topic Microbiology
General
Microbiology
url https://pubmed.ncbi.nlm.nih.gov/40414713/?utm_source=Chrome&utm_medium=rss&utm_campaign=None&utm_content=12ukGnx_-plPNzlmyrXx8F1eFGQoACErVTYjKOgfuFGEA7Ys2x&fc=None&ff=20260606085540&v=2.20.0