Full Text Available
Note: Clicking the button above will open the full text document at the original institutional repository in a new window.
The effect of Ephedra foeminea on human bone osteosarcoma U2OS cell viability and migration
Although advancement has been made in the development of cancer treatments, contemporary treatments still present significant challenges such as low effectiveness and adverse side effects. There is thus a critical need to continuously develop new and more effective drugs against cancer. Herbal plant...
Saved in:
| Main Author: | |
|---|---|
| Format: | Thesis |
| Published: |
AUC Knowledge Fountain
2019
|
| Subjects: | |
| Tags: |
No Tags, Be the first to tag this record!
|
| _version_ | 1867613411859234816 |
|---|---|
| access_status_str | Open Access |
| author | Mpingirika, Eric Zadok |
| author_browse | Mpingirika, Eric Zadok |
| author_facet | Mpingirika, Eric Zadok |
| author_sort | Mpingirika, Eric Zadok |
| collection | Thesis |
| dc_rights_str_mv | The author retains all rights with regard to copyright. The author certifies that written permission from the owner(s) of third-party copyrighted matter included in the thesis, dissertation, paper, or record of study has been obtained. The author further certifies that IRB approval has been obtained for this thesis, or that IRB approval is not necessary for this thesis. Insofar as this thesis, dissertation, paper, or record of study is an educational record as defined in the Family Educational Rights and Privacy Act (FERPA) (20 USC 1232g), the author has granted consent to disclosure of it to anyone who requests a copy. |
| description | Although advancement has been made in the development of cancer treatments, contemporary treatments still present significant challenges such as low effectiveness and adverse side effects. There is thus a critical need to continuously develop new and more effective drugs against cancer. Herbal plants serve as a potential source for a wide variety of complex compounds with probable anticancer activity. E. foeminea is an herb whose use in the Middle East recently gained popularity as a remedy for cancer. There is however minimal empirical evidence regarding the anticancer effects of E. foeminea. In this study, the effect of E.foeminea ethyl acetate, ethanol and water extracts on morphology, viability, migratory ability and gene expression of U2OS osteosarcoma cells was examined. U2OS viability, migratory ability and the steady-state mRNA levels of genes involved in these processes were respectively studied using MTT assay, wound healing assay and reverse transcriptase PCR (RT-PCR). Results showed that all tested extracts significantly reduced U2OS percentage viability in a manner dependent on both dose and time with varying potencies; the least half maximal inhibitory concentration (IC50) recorded was that of the water extract after 48h incubation (30.761±1.4 μg/ml) followed by the ethyl acetate extract after 72h incubation (80.35±1.233 μg/ml) and finally the ethanol extract after 48h incubation (97.499±1.188 μg/ml). Ethanol extract significantly reduced U2OS percentage wound closure while both ethanol and water extracts significantly reduced the steady-state mRNA expression of Beta-catenin and its downstream targets, Twist1 and RUNX2, which are critical in promoting both proliferation and cell migration in osteosarcoma. These results suggest that E. foeminea decreases U2OS cell viability and migration by modulating the expression of key genes involved in regulating these processes. |
| format | Thesis |
| id | oai:fount.aucegypt.edu:etds-1721 |
| institution | American University in Cairo (Egypt) |
| last_indexed | 2026-06-10T12:35:43.583Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from AUC Knowledge Fountain — bepress |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | AUC Knowledge Fountain |
| publisherStr | AUC Knowledge Fountain |
| record_format | dspace |
| source_str | AUC Knowledge Fountain — bepress |
| spelling | oai:fount.aucegypt.edu:etds-1721 The effect of Ephedra foeminea on human bone osteosarcoma U2OS cell viability and migration Mpingirika, Eric Zadok Although advancement has been made in the development of cancer treatments, contemporary treatments still present significant challenges such as low effectiveness and adverse side effects. There is thus a critical need to continuously develop new and more effective drugs against cancer. Herbal plants serve as a potential source for a wide variety of complex compounds with probable anticancer activity. E. foeminea is an herb whose use in the Middle East recently gained popularity as a remedy for cancer. There is however minimal empirical evidence regarding the anticancer effects of E. foeminea. In this study, the effect of E.foeminea ethyl acetate, ethanol and water extracts on morphology, viability, migratory ability and gene expression of U2OS osteosarcoma cells was examined. U2OS viability, migratory ability and the steady-state mRNA levels of genes involved in these processes were respectively studied using MTT assay, wound healing assay and reverse transcriptase PCR (RT-PCR). Results showed that all tested extracts significantly reduced U2OS percentage viability in a manner dependent on both dose and time with varying potencies; the least half maximal inhibitory concentration (IC50) recorded was that of the water extract after 48h incubation (30.761±1.4 μg/ml) followed by the ethyl acetate extract after 72h incubation (80.35±1.233 μg/ml) and finally the ethanol extract after 48h incubation (97.499±1.188 μg/ml). Ethanol extract significantly reduced U2OS percentage wound closure while both ethanol and water extracts significantly reduced the steady-state mRNA expression of Beta-catenin and its downstream targets, Twist1 and RUNX2, which are critical in promoting both proliferation and cell migration in osteosarcoma. These results suggest that E. foeminea decreases U2OS cell viability and migration by modulating the expression of key genes involved in regulating these processes. 2019-02-01T08:00:00Z thesis text/html https://fount.aucegypt.edu/etds/722 https://fount.aucegypt.edu/context/etds/article/1721/type/native/viewcontent/_28F_29Mpingirika_20Eric__20Final_20Thesis_5_Jan_2018_AA_MEZ.pdf_sequence_3 The author retains all rights with regard to copyright. The author certifies that written permission from the owner(s) of third-party copyrighted matter included in the thesis, dissertation, paper, or record of study has been obtained. The author further certifies that IRB approval has been obtained for this thesis, or that IRB approval is not necessary for this thesis. Insofar as this thesis, dissertation, paper, or record of study is an educational record as defined in the Family Educational Rights and Privacy Act (FERPA) (20 USC 1232g), the author has granted consent to disclosure of it to anyone who requests a copy. Theses and Dissertations AUC Knowledge Fountain Ephedra Ephedra foeminea |
| spellingShingle | Ephedra Ephedra foeminea Mpingirika, Eric Zadok The effect of Ephedra foeminea on human bone osteosarcoma U2OS cell viability and migration |
| title | The effect of Ephedra foeminea on human bone osteosarcoma U2OS cell viability and migration |
| title_full | The effect of Ephedra foeminea on human bone osteosarcoma U2OS cell viability and migration |
| title_fullStr | The effect of Ephedra foeminea on human bone osteosarcoma U2OS cell viability and migration |
| title_full_unstemmed | The effect of Ephedra foeminea on human bone osteosarcoma U2OS cell viability and migration |
| title_short | The effect of Ephedra foeminea on human bone osteosarcoma U2OS cell viability and migration |
| title_sort | effect of ephedra foeminea on human bone osteosarcoma u2os cell viability and migration |
| topic | Ephedra Ephedra foeminea |
| url | https://fount.aucegypt.edu/etds/722 https://fount.aucegypt.edu/context/etds/article/1721/type/native/viewcontent/_28F_29Mpingirika_20Eric__20Final_20Thesis_5_Jan_2018_AA_MEZ.pdf_sequence_3 |
| work_keys_str_mv | AT mpingirikaericzadok theeffectofephedrafoemineaonhumanboneosteosarcomau2oscellviabilityandmigration AT mpingirikaericzadok effectofephedrafoemineaonhumanboneosteosarcomau2oscellviabilityandmigration |