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The Antioxidant and Antimicrobial Activities of Cornsilk Extract Encapsulated in Polysaccharide Nanoparticles
Corn silk possess antibacterial and antioxidant activities due to its phenolic compounds but they are of low bioavailability that requires some innovative techniques to develop such active ingredients in a safe and less toxic manner. Accordingly, silk extract SE was encapsulated in chitosan nanopart...
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2021
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| access_status_str | Open Access |
| author | Mady, Maryam |
| author_browse | Mady, Maryam |
| author_facet | Mady, Maryam |
| author_sort | Mady, Maryam |
| collection | Thesis |
| dc_rights_str_mv | The American University in Cairo grants authors of theses and dissertations a maximum embargo period of two years from the date of submission, upon request. After the embargo elapses, these documents are made available publicly. If you are the author of this thesis or dissertation, and would like to request an exceptional extension of the embargo period, please write to thesisadmin@aucegypt.edu http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| description | Corn silk possess antibacterial and antioxidant activities due to its phenolic compounds but they are of low bioavailability that requires some innovative techniques to develop such active ingredients in a safe and less toxic manner. Accordingly, silk extract SE was encapsulated in chitosan nanoparticles CS NPs, which is a natural and abundant polymer derived from chitin. Among 15 experimental trials via design expert software, the optimum formula of SE x\CS NPs was prepared at 800 rpm, 5hrs. on magnetic stirrer, and by CS: TPP ratio was 2.5:1. Then SEM showed the majority of particles size diameter in the range of 25, 37, 39, and 55 and 77 nm were confirmatory for DLS particle size measurements of SE 0.5\CS NPs, SE 1\CS NPs, SE 1.5\CS NPs, and SE 2\CS NPs. FTIR results were confirmed the successful encapsulation and of SE in CS NPs by the appearance of the aromatic group bands at the wavenumber 1577.9 and 1417.5 cm-1. The cumulative extract release percentage was compared in two PH media showing no difference in their release profile. The release behavior of SE from CS NPs in those PH was indicative for using SE x\CS NPs as a topical preparation. SE x\CS NPs were tested for their antioxidant activities where % inhibition (free radical scavenging activity) of the four preparations SE 0.5\CS NPs, SE 1\CS NPs, SE 1.5\CS NPs, and SE 2\CS NPs showed 83.7, 84.7, 87.4, and 95.4% respectively higher than their corresponding amounts of free extract 31.5, 61.1, 74, and 87.4%. The TPC assay showed much lesser results for the four preparations of SE x\CS NPs ranged from 2.6 to 3.7 mg GAE\gm sample than that of their corresponding amounts of free extract which ranged from 16.5 to 35.7 mg GAE\gm sample. Also, the decreased values of TPC was indicative for the successful encapsulation of SE. The antibacterial test against S.auerus showed 100% BGI. In contrary, the antibacterial test against E.coli for the four preparations SE 0.5\CS NPs, SE 1\CS NPs, SE 1.5\CS NPs, and SE 2\CS NPs showed 70.44, 76.10, 82.51, and 98.27% BGI which was higher than that of their corresponding amounts of free extract 40.11, 59.30, 73.40, and 84.01% BGI. |
| format | Thesis |
| id | oai:fount.aucegypt.edu:etds-2534 |
| institution | American University in Cairo (Egypt) |
| last_indexed | 2026-06-10T12:35:50.652Z |
| license_str | Creative Commons |
| provenance_str_mv | Harvested via OAI-PMH from AUC Knowledge Fountain — bepress |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | AUC Knowledge Fountain |
| publisherStr | AUC Knowledge Fountain |
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| source_str | AUC Knowledge Fountain — bepress |
| spelling | oai:fount.aucegypt.edu:etds-2534 The Antioxidant and Antimicrobial Activities of Cornsilk Extract Encapsulated in Polysaccharide Nanoparticles Mady, Maryam Corn silk possess antibacterial and antioxidant activities due to its phenolic compounds but they are of low bioavailability that requires some innovative techniques to develop such active ingredients in a safe and less toxic manner. Accordingly, silk extract SE was encapsulated in chitosan nanoparticles CS NPs, which is a natural and abundant polymer derived from chitin. Among 15 experimental trials via design expert software, the optimum formula of SE x\CS NPs was prepared at 800 rpm, 5hrs. on magnetic stirrer, and by CS: TPP ratio was 2.5:1. Then SEM showed the majority of particles size diameter in the range of 25, 37, 39, and 55 and 77 nm were confirmatory for DLS particle size measurements of SE 0.5\CS NPs, SE 1\CS NPs, SE 1.5\CS NPs, and SE 2\CS NPs. FTIR results were confirmed the successful encapsulation and of SE in CS NPs by the appearance of the aromatic group bands at the wavenumber 1577.9 and 1417.5 cm-1. The cumulative extract release percentage was compared in two PH media showing no difference in their release profile. The release behavior of SE from CS NPs in those PH was indicative for using SE x\CS NPs as a topical preparation. SE x\CS NPs were tested for their antioxidant activities where % inhibition (free radical scavenging activity) of the four preparations SE 0.5\CS NPs, SE 1\CS NPs, SE 1.5\CS NPs, and SE 2\CS NPs showed 83.7, 84.7, 87.4, and 95.4% respectively higher than their corresponding amounts of free extract 31.5, 61.1, 74, and 87.4%. The TPC assay showed much lesser results for the four preparations of SE x\CS NPs ranged from 2.6 to 3.7 mg GAE\gm sample than that of their corresponding amounts of free extract which ranged from 16.5 to 35.7 mg GAE\gm sample. Also, the decreased values of TPC was indicative for the successful encapsulation of SE. The antibacterial test against S.auerus showed 100% BGI. In contrary, the antibacterial test against E.coli for the four preparations SE 0.5\CS NPs, SE 1\CS NPs, SE 1.5\CS NPs, and SE 2\CS NPs showed 70.44, 76.10, 82.51, and 98.27% BGI which was higher than that of their corresponding amounts of free extract 40.11, 59.30, 73.40, and 84.01% BGI. 2021-01-31T08:00:00Z thesis application/pdf https://fount.aucegypt.edu/etds/1526 https://fount.aucegypt.edu/context/etds/article/2534/viewcontent/MADY_maryam_thesis_2020.pdf The American University in Cairo grants authors of theses and dissertations a maximum embargo period of two years from the date of submission, upon request. After the embargo elapses, these documents are made available publicly. If you are the author of this thesis or dissertation, and would like to request an exceptional extension of the embargo period, please write to thesisadmin@aucegypt.edu http://creativecommons.org/licenses/by-nc-nd/4.0/ Theses and Dissertations AUC Knowledge Fountain Cornsilk Chitosan Nanoparticles Antioxidant Antibacterial Chemicals and Drugs Nanotechnology Natural Products Chemistry and Pharmacognosy Pharmacy and Pharmaceutical Sciences |
| spellingShingle | Cornsilk Chitosan Nanoparticles Antioxidant Antibacterial Chemicals and Drugs Nanotechnology Natural Products Chemistry and Pharmacognosy Pharmacy and Pharmaceutical Sciences Mady, Maryam The Antioxidant and Antimicrobial Activities of Cornsilk Extract Encapsulated in Polysaccharide Nanoparticles |
| title | The Antioxidant and Antimicrobial Activities of Cornsilk Extract Encapsulated in Polysaccharide Nanoparticles |
| title_full | The Antioxidant and Antimicrobial Activities of Cornsilk Extract Encapsulated in Polysaccharide Nanoparticles |
| title_fullStr | The Antioxidant and Antimicrobial Activities of Cornsilk Extract Encapsulated in Polysaccharide Nanoparticles |
| title_full_unstemmed | The Antioxidant and Antimicrobial Activities of Cornsilk Extract Encapsulated in Polysaccharide Nanoparticles |
| title_short | The Antioxidant and Antimicrobial Activities of Cornsilk Extract Encapsulated in Polysaccharide Nanoparticles |
| title_sort | antioxidant and antimicrobial activities of cornsilk extract encapsulated in polysaccharide nanoparticles |
| topic | Cornsilk Chitosan Nanoparticles Antioxidant Antibacterial Chemicals and Drugs Nanotechnology Natural Products Chemistry and Pharmacognosy Pharmacy and Pharmaceutical Sciences |
| url | https://fount.aucegypt.edu/etds/1526 https://fount.aucegypt.edu/context/etds/article/2534/viewcontent/MADY_maryam_thesis_2020.pdf |
| work_keys_str_mv | AT madymaryam theantioxidantandantimicrobialactivitiesofcornsilkextractencapsulatedinpolysaccharidenanoparticles AT madymaryam antioxidantandantimicrobialactivitiesofcornsilkextractencapsulatedinpolysaccharidenanoparticles |