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Comparative Metabolites Profiling of Sesamum indicum Seeds in Context of their Origin, Processing and Biological Activity Using Bioinformatics Tools
Sesame (Sesamum indicum), is a significant oilseed crop with great economical and nutritional value, encompassing a plethora of primary and secondary metabolites that exhibit pharmacological and therapeutic merits. The present study, using untargeted comparative metabolomics, aims to authenticate an...
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2022
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| _version_ | 1867613421937098752 |
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| access_status_str | Open Access |
| author | Abib, Bishoy Fayez |
| author_browse | Abib, Bishoy Fayez |
| author_facet | Abib, Bishoy Fayez |
| author_sort | Abib, Bishoy Fayez |
| collection | Thesis |
| description | Sesame (Sesamum indicum), is a significant oilseed crop with great economical and nutritional value, encompassing a plethora of primary and secondary metabolites that exhibit pharmacological and therapeutic merits. The present study, using untargeted comparative metabolomics, aims to authenticate and classify sesame seed accessions from different countries of origin i.e., Egypt, Sudan and Nigeria, based on their metabolite variations and in relation to their biological activity and nutritional value such as mineral content. Also, to further assess the impact of processing on sesame’s nutritional value and biological activity using the industrial production of sesame paste as a case study. To achieve such results, data sets generated from different analytical platforms GC-MS post-silylation, HS-SPME-GC-MS and UPLC-MS were coupled to chemometrics to aid in providing chemical information for better quality control and nutritional assessments. The identified metabolite profiles for raw accessions and processed sesame samples belonged to a myriad of chemical classes including sugars, sugar alcohols and acids, polyphenolic compounds, amino acids, nitrogenous compounds, fatty acids, fatty alcohols, aldehydes, ketones, esters, phytosterols, heterocyclic compounds and organic acids. GC-MS analysis comprising 85 peaks for raw accessions and 114 peaks for processed samples, in addition to HS-SPME results comprising 134 peaks for raw and laboratory-roasted seeds, revealed variation in fatty acid composition among raw sesame accessions, such as Nigerian sesame, which was abundant in oleic and linoleic fatty acids. Moreover, a total of 139 peaks were identified using UPLC-MS, which lead to the tentative identification of novel compounds in sesame i.e., 1- linoleoylglycerophosphoinositol, 1-palmitoylglycerophosphoinositol and sesaminol 2,6- dicatechol monomethyl ether. Discriminatory markers based on UPLC-MS data belonging to coumarins, lignans, phenolic and organic acids were also revealed among raw accessions, whereas roasted and unroasted seeds were distinguished by their sugar, peptide/amino acid, and organic acid contents. Furthermore, the mineral content of sesame seeds revealed the negative impact of processing on its overall content, and the impact of cultivation region, which was indicated by the low mineral content in Egyptian sesame compared to others. The antioxidant capacity assays also revealed strong scavenging activity for raw accessions in DPPH and β-carotene-lineolate assays and declined antioxidant capacity as a result of processing |
| format | Thesis |
| id | oai:fount.aucegypt.edu:etds-3010 |
| institution | American University in Cairo (Egypt) |
| last_indexed | 2026-06-10T12:35:53.165Z |
| license_str | Not specified — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from AUC Knowledge Fountain — bepress |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | AUC Knowledge Fountain |
| publisherStr | AUC Knowledge Fountain |
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| source_str | AUC Knowledge Fountain — bepress |
| spelling | oai:fount.aucegypt.edu:etds-3010 Comparative Metabolites Profiling of Sesamum indicum Seeds in Context of their Origin, Processing and Biological Activity Using Bioinformatics Tools Abib, Bishoy Fayez Sesame (Sesamum indicum), is a significant oilseed crop with great economical and nutritional value, encompassing a plethora of primary and secondary metabolites that exhibit pharmacological and therapeutic merits. The present study, using untargeted comparative metabolomics, aims to authenticate and classify sesame seed accessions from different countries of origin i.e., Egypt, Sudan and Nigeria, based on their metabolite variations and in relation to their biological activity and nutritional value such as mineral content. Also, to further assess the impact of processing on sesame’s nutritional value and biological activity using the industrial production of sesame paste as a case study. To achieve such results, data sets generated from different analytical platforms GC-MS post-silylation, HS-SPME-GC-MS and UPLC-MS were coupled to chemometrics to aid in providing chemical information for better quality control and nutritional assessments. The identified metabolite profiles for raw accessions and processed sesame samples belonged to a myriad of chemical classes including sugars, sugar alcohols and acids, polyphenolic compounds, amino acids, nitrogenous compounds, fatty acids, fatty alcohols, aldehydes, ketones, esters, phytosterols, heterocyclic compounds and organic acids. GC-MS analysis comprising 85 peaks for raw accessions and 114 peaks for processed samples, in addition to HS-SPME results comprising 134 peaks for raw and laboratory-roasted seeds, revealed variation in fatty acid composition among raw sesame accessions, such as Nigerian sesame, which was abundant in oleic and linoleic fatty acids. Moreover, a total of 139 peaks were identified using UPLC-MS, which lead to the tentative identification of novel compounds in sesame i.e., 1- linoleoylglycerophosphoinositol, 1-palmitoylglycerophosphoinositol and sesaminol 2,6- dicatechol monomethyl ether. Discriminatory markers based on UPLC-MS data belonging to coumarins, lignans, phenolic and organic acids were also revealed among raw accessions, whereas roasted and unroasted seeds were distinguished by their sugar, peptide/amino acid, and organic acid contents. Furthermore, the mineral content of sesame seeds revealed the negative impact of processing on its overall content, and the impact of cultivation region, which was indicated by the low mineral content in Egyptian sesame compared to others. The antioxidant capacity assays also revealed strong scavenging activity for raw accessions in DPPH and β-carotene-lineolate assays and declined antioxidant capacity as a result of processing 2022-11-30T08:00:00Z thesis application/pdf https://fount.aucegypt.edu/etds/1977 https://fount.aucegypt.edu/context/etds/article/3010/viewcontent/Bishoy_Fayez_Abib_Thesis_2.pdf Theses and Dissertations AUC Knowledge Fountain Metabolomics Sesamum indicumn Antioxidant Activity Multivariate Data Analysis UPLC-MS Secondary Metabolites Dietary Minerals GC-MS HS-SPME. Agricultural Science Agronomy and Crop Sciences Analytical Chemistry Biochemistry Bioinformatics Comparative Nutrition Food Chemistry Food Processing Other Genetics and Genomics Other Nutrition Other Plant Sciences |
| spellingShingle | Metabolomics Sesamum indicumn Antioxidant Activity Multivariate Data Analysis UPLC-MS Secondary Metabolites Dietary Minerals GC-MS HS-SPME. Agricultural Science Agronomy and Crop Sciences Analytical Chemistry Biochemistry Bioinformatics Comparative Nutrition Food Chemistry Food Processing Other Genetics and Genomics Other Nutrition Other Plant Sciences Abib, Bishoy Fayez Comparative Metabolites Profiling of Sesamum indicum Seeds in Context of their Origin, Processing and Biological Activity Using Bioinformatics Tools |
| title | Comparative Metabolites Profiling of Sesamum indicum Seeds in Context of their Origin, Processing and Biological Activity Using Bioinformatics Tools |
| title_full | Comparative Metabolites Profiling of Sesamum indicum Seeds in Context of their Origin, Processing and Biological Activity Using Bioinformatics Tools |
| title_fullStr | Comparative Metabolites Profiling of Sesamum indicum Seeds in Context of their Origin, Processing and Biological Activity Using Bioinformatics Tools |
| title_full_unstemmed | Comparative Metabolites Profiling of Sesamum indicum Seeds in Context of their Origin, Processing and Biological Activity Using Bioinformatics Tools |
| title_short | Comparative Metabolites Profiling of Sesamum indicum Seeds in Context of their Origin, Processing and Biological Activity Using Bioinformatics Tools |
| title_sort | comparative metabolites profiling of sesamum indicum seeds in context of their origin processing and biological activity using bioinformatics tools |
| topic | Metabolomics Sesamum indicumn Antioxidant Activity Multivariate Data Analysis UPLC-MS Secondary Metabolites Dietary Minerals GC-MS HS-SPME. Agricultural Science Agronomy and Crop Sciences Analytical Chemistry Biochemistry Bioinformatics Comparative Nutrition Food Chemistry Food Processing Other Genetics and Genomics Other Nutrition Other Plant Sciences |
| url | https://fount.aucegypt.edu/etds/1977 https://fount.aucegypt.edu/context/etds/article/3010/viewcontent/Bishoy_Fayez_Abib_Thesis_2.pdf |
| work_keys_str_mv | AT abibbishoyfayez comparativemetabolitesprofilingofsesamumindicumseedsincontextoftheiroriginprocessingandbiologicalactivityusingbioinformaticstools |