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The enzymatic release of glycosidically-bound terpenes in must
Bibliography: pages 79-86.
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| Other Authors: | |
| Format: | Thesis |
| Language: | English |
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Department of Molecular and Cell Biology
2016
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| _version_ | 1867613264846782464 |
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| access_status_str | Open Access |
| author | Strauss, Lindie Helene |
| author2 | Botes, DP |
| author_browse | Botes, DP Strauss, Lindie Helene |
| author_facet | Botes, DP Strauss, Lindie Helene |
| author_sort | Strauss, Lindie Helene |
| collection | Thesis |
| description | Bibliography: pages 79-86. |
| format | Thesis |
| id | oai:open.uct.ac.za:11427/22185 |
| institution | University of Cape Town (South Africa) |
| language | eng |
| last_indexed | 2026-06-10T12:33:23.204Z |
| license_str | Not specified — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UCTD — University of Cape Town Open Access Repository |
| publishDate | 2016 |
| publishDateRange | 2016 |
| publishDateSort | 2016 |
| publisher | Department of Molecular and Cell Biology |
| publisherStr | Department of Molecular and Cell Biology |
| record_format | dspace |
| source_str | UCTD — University of Cape Town Open Access Repository |
| spelling | oai:open.uct.ac.za:11427/22185 The enzymatic release of glycosidically-bound terpenes in must Strauss, Lindie Helene Botes, DP Terpenes Must Enzymes Bibliography: pages 79-86. Flavour in wines is perhaps the most important factor affecting wine quality, with monoterpenoids being among the compounds contributing to flavour. In grapes there exists glycosidically-bound forms of these monoterpenes, representing a latent source of aroma. This project is a study on the ability of different enzymes to release these monoterpenes in Muscat d'Alexandrie and Weisser Riesling grapes. Different commercial enzyme preparations were characterised with respect to their catalytic properties and fractionated by ion exchange chromatography in the most active fractions. Amongst those commercial preparations tested, Rohapect C was found to be the most effective at conditions prevalent during wine making. These purified fractions were added to the above mentioned grapes to determine their effect on the release of different monoterpenes. Even though the enzymes were active on synthetic substrates, limited release of terpenes from must could be detected. Possible causes for this apparent inability to release monoterpenes were investigated. Product inhibition due to the presence of high concentrations of glucose in the must appeared to be the main cause of limited enzyme activity. In an attempt to overcome this problem, glucose in the above mentioned cultivars was oxidised to gluconic acid by glucose oxidase, prior to attempting monoterpene release by Rohapect C. Although no marked increase in the release of total terpenes occured, a significant increase in the concentrations of some individual terpenes could be observed. The effect of this on wine quality remains to be ascertained. 2016-10-19T03:55:42Z 2016-10-19T03:55:42Z 1989 Master Thesis Masters MSc http://hdl.handle.net/11427/22185 eng application/pdf Department of Molecular and Cell Biology Faculty of Science University of Cape Town |
| spellingShingle | Terpenes Must Enzymes Strauss, Lindie Helene The enzymatic release of glycosidically-bound terpenes in must |
| thesis_degree_str | Master's |
| title | The enzymatic release of glycosidically-bound terpenes in must |
| title_full | The enzymatic release of glycosidically-bound terpenes in must |
| title_fullStr | The enzymatic release of glycosidically-bound terpenes in must |
| title_full_unstemmed | The enzymatic release of glycosidically-bound terpenes in must |
| title_short | The enzymatic release of glycosidically-bound terpenes in must |
| title_sort | enzymatic release of glycosidically bound terpenes in must |
| topic | Terpenes Must Enzymes |
| url | http://hdl.handle.net/11427/22185 |
| work_keys_str_mv | AT strausslindiehelene theenzymaticreleaseofglycosidicallyboundterpenesinmust AT strausslindiehelene enzymaticreleaseofglycosidicallyboundterpenesinmust |