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Rat liver nuclear envelope insulin binding and its effects on endogenous protein kinases
The postulated model for the insulin - stimulated induction of mRNA efflux (Purrello et al., 1983) is based on the demonstrated binding of insulin to intracellular membrane structures (see chapter 2, section 2.2.1), and the in vitro effect of insulin on nuclear envelope phosphorylation, NTPase activ...
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| Format: | Thesis |
| Language: | English English |
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Department of Molecular and Cell Biology
2017
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| _version_ | 1867613225360556034 |
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| access_status_str | Open Access |
| author | Sabbatini, G P Sabbatini, G P |
| author2 | von Holt, C |
| author_browse | Sabbatini, G P von Holt, C |
| author_facet | von Holt, C Sabbatini, G P Sabbatini, G P |
| author_sort | Sabbatini, G P |
| collection | Thesis |
| description | The postulated model for the insulin - stimulated induction of mRNA efflux (Purrello et al., 1983) is based on the demonstrated binding of insulin to intracellular membrane structures (see chapter 2, section 2.2.1), and the in vitro effect of insulin on nuclear envelope phosphorylation, NTPase activity, and mRNA efflux (see chapter 5, section 5.1). These independent observations have led to the development of a model for the direct induction by insulin, at the level of the nucleus, of mRNA efflux (figure 1.1). However, the specific intracellular insulin binding has been inf erred from kinetic or morphological studies which have not identified a discrete membrane - bound polypeptide(s) as an insulin docking molecule in situ (Goldfine, 1981). Also, the stimulation of NTPase activity has only been established by monitoring the level of general ATP hydrolysis of nuclear envelope fractions in the presence and absence of insulin (Purrello et al., 1983). The scope of this thesis has been to further the understanding of this mechanism by attempting to a) unequivocally identify a specific nuclear envelope - associated insulin docking polypeptide in situ and b) to demonstrate that insulin directly affects the ATP - binding of nuclear envelope ATP - binding proteins. The latter would demonstrate a primary effect of insulin i.e. the modulation of the ATP - binding capacity of identified NTPases / protein kinases (or their release from some inactive storage form), and not a general phenomenon such as elevated ATP. |
| format | Thesis |
| id | oai:open.uct.ac.za:11427/23361 |
| institution | University of Cape Town (South Africa) |
| language | eng eng |
| last_indexed | 2026-06-10T12:32:45.765Z |
| license_str | Not specified — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UCTD — University of Cape Town Open Access Repository |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| publisher | Department of Molecular and Cell Biology |
| publisherStr | Department of Molecular and Cell Biology |
| record_format | dspace |
| source_str | UCTD — University of Cape Town Open Access Repository |
| spelling | oai:open.uct.ac.za:11427/23361 Rat liver nuclear envelope insulin binding and its effects on endogenous protein kinases Rat liver nuclear envelope insulin binding and its effects on endogenous protein kinases Sabbatini, G P Sabbatini, G P von Holt, C Von Holt, Claus Molecular and Cell Biology The postulated model for the insulin - stimulated induction of mRNA efflux (Purrello et al., 1983) is based on the demonstrated binding of insulin to intracellular membrane structures (see chapter 2, section 2.2.1), and the in vitro effect of insulin on nuclear envelope phosphorylation, NTPase activity, and mRNA efflux (see chapter 5, section 5.1). These independent observations have led to the development of a model for the direct induction by insulin, at the level of the nucleus, of mRNA efflux (figure 1.1). However, the specific intracellular insulin binding has been inf erred from kinetic or morphological studies which have not identified a discrete membrane - bound polypeptide(s) as an insulin docking molecule in situ (Goldfine, 1981). Also, the stimulation of NTPase activity has only been established by monitoring the level of general ATP hydrolysis of nuclear envelope fractions in the presence and absence of insulin (Purrello et al., 1983). The scope of this thesis has been to further the understanding of this mechanism by attempting to a) unequivocally identify a specific nuclear envelope - associated insulin docking polypeptide in situ and b) to demonstrate that insulin directly affects the ATP - binding of nuclear envelope ATP - binding proteins. The latter would demonstrate a primary effect of insulin i.e. the modulation of the ATP - binding capacity of identified NTPases / protein kinases (or their release from some inactive storage form), and not a general phenomenon such as elevated ATP. 2017-01-26T11:32:34Z 2017-01-26T11:32:34Z 1992 2016-11-22T10:49:31Z Doctoral Thesis Doctoral PhD http://hdl.handle.net/11427/23361 eng eng application/pdf Department of Molecular and Cell Biology Faculty of Science University of Cape Town |
| spellingShingle | Molecular and Cell Biology Sabbatini, G P Sabbatini, G P Rat liver nuclear envelope insulin binding and its effects on endogenous protein kinases |
| thesis_degree_str | Doctoral |
| title | Rat liver nuclear envelope insulin binding and its effects on endogenous protein kinases |
| title_full | Rat liver nuclear envelope insulin binding and its effects on endogenous protein kinases |
| title_fullStr | Rat liver nuclear envelope insulin binding and its effects on endogenous protein kinases |
| title_full_unstemmed | Rat liver nuclear envelope insulin binding and its effects on endogenous protein kinases |
| title_short | Rat liver nuclear envelope insulin binding and its effects on endogenous protein kinases |
| title_sort | rat liver nuclear envelope insulin binding and its effects on endogenous protein kinases |
| topic | Molecular and Cell Biology |
| url | http://hdl.handle.net/11427/23361 |
| work_keys_str_mv | AT sabbatinigp ratlivernuclearenvelopeinsulinbindinganditseffectsonendogenousproteinkinases AT sabbatinigp ratlivernuclearenvelopeinsulinbindinganditseffectsonendogenousproteinkinases |