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An investigation into the biocatalytic application of the thermostable nitrile hydratase from the thermophilic strain Geobacillus pallidus RAPc8
Includes abstract.
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| Other Authors: | |
| Format: | Thesis |
| Language: | English |
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Department of Chemical Engineering
2014
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| _version_ | 1867613249302691840 |
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| access_status_str | Open Access |
| author | Chiyanzu, Idan |
| author2 | Burton, Stephanie Gail |
| author_browse | Burton, Stephanie Gail Chiyanzu, Idan |
| author_facet | Burton, Stephanie Gail Chiyanzu, Idan |
| author_sort | Chiyanzu, Idan |
| collection | Thesis |
| description | Includes abstract. |
| format | Thesis |
| id | oai:open.uct.ac.za:11427/5367 |
| institution | University of Cape Town (South Africa) |
| language | eng |
| last_indexed | 2026-06-10T12:33:08.525Z |
| license_str | Not specified — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UCTD — University of Cape Town Open Access Repository |
| publishDate | 2014 |
| publishDateRange | 2014 |
| publishDateSort | 2014 |
| publisher | Department of Chemical Engineering |
| publisherStr | Department of Chemical Engineering |
| record_format | dspace |
| source_str | UCTD — University of Cape Town Open Access Repository |
| spelling | oai:open.uct.ac.za:11427/5367 An investigation into the biocatalytic application of the thermostable nitrile hydratase from the thermophilic strain Geobacillus pallidus RAPc8 Chiyanzu, Idan Burton, Stephanie Gail Chemical Engineering Includes abstract. Includes bibliographical references (leaves 190-215). Nitrile hydratases (NHases) are bacterial metalloenzymes that catalyze the hydration of nitriles to their corresponding amides. The enzymes have been found in several microorganisms and participate in the metabolism of nitrile compounds as source of carbon and nitrogen. The commercial use of NHases is well recognized and has prompted research interest as well as the application of the enzymes in the manufacture of commodity amide chemicals. This has largely been due to the versatile nature of the enzymes, associated with their physiochemical properties and broad substrate specificity. However, the widespread application of nitrile-converting enzymes in the industrial processes has been restricted in part by the thermal instability of the mesophilic-derived enzymes, and thus there is an increased focus on NHases from thermophilic microorganisms. A novel moderately thermophilic microorganism, Geobacillus pallidus RAPc8, was isolated by our collaborators (Pereira and co-workers, 1998). The strain has an optimal growth temperature of 65oC and constutitively expresses a thermostable nitrile hydratase. The gene cluster containing the nitrile hydratase were cloned, sequenced, and inducibly expressed in E. coli BL21 (DE3) to levels of approximately 49 U/mg. The NHase was purified by four steps including heat treatment, ammonium sulfate precipitation, hydrophobic interaction chromatography and ion exchange chromatography. 2014-07-31T11:13:13Z 2014-07-31T11:13:13Z 2008 Doctoral Thesis Doctoral PhD http://hdl.handle.net/11427/5367 eng application/pdf Department of Chemical Engineering Faculty of Engineering and the Built Environment University of Cape Town |
| spellingShingle | Chemical Engineering Chiyanzu, Idan An investigation into the biocatalytic application of the thermostable nitrile hydratase from the thermophilic strain Geobacillus pallidus RAPc8 |
| thesis_degree_str | Doctoral |
| title | An investigation into the biocatalytic application of the thermostable nitrile hydratase from the thermophilic strain Geobacillus pallidus RAPc8 |
| title_full | An investigation into the biocatalytic application of the thermostable nitrile hydratase from the thermophilic strain Geobacillus pallidus RAPc8 |
| title_fullStr | An investigation into the biocatalytic application of the thermostable nitrile hydratase from the thermophilic strain Geobacillus pallidus RAPc8 |
| title_full_unstemmed | An investigation into the biocatalytic application of the thermostable nitrile hydratase from the thermophilic strain Geobacillus pallidus RAPc8 |
| title_short | An investigation into the biocatalytic application of the thermostable nitrile hydratase from the thermophilic strain Geobacillus pallidus RAPc8 |
| title_sort | investigation into the biocatalytic application of the thermostable nitrile hydratase from the thermophilic strain geobacillus pallidus rapc8 |
| topic | Chemical Engineering |
| url | http://hdl.handle.net/11427/5367 |
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