Full Text Available
Note: Clicking the button above will open the full text document at the original institutional repository in a new window.
Characterization of a thermostable amidase and development of a bioreactor process for lactic acid production
This thesis reports studies of the biochemical properties of a newly isolated thermostable amidase from Bacillus sp. RAPc8, and development of a continuous reactor process for the production of a target product. The amidase was cloned and over-expressed in E. coli BL21 strain pNH 223 pLySs by Camero...
Saved in:
| Main Author: | |
|---|---|
| Other Authors: | |
| Format: | Thesis |
| Language: | English |
| Published: |
Centre for Bioprocess Engineering Research
2014
|
| Subjects: | |
| Tags: |
No Tags, Be the first to tag this record!
|
| _version_ | 1867613322161946624 |
|---|---|
| access_status_str | Open Access |
| author | Makhongela, Happy Steven |
| author2 | Burton, Stephanie Gail |
| author_browse | Burton, Stephanie Gail Makhongela, Happy Steven |
| author_facet | Burton, Stephanie Gail Makhongela, Happy Steven |
| author_sort | Makhongela, Happy Steven |
| collection | Thesis |
| description | This thesis reports studies of the biochemical properties of a newly isolated thermostable amidase from Bacillus sp. RAPc8, and development of a continuous reactor process for the production of a target product. The amidase was cloned and over-expressed in E. coli BL21 strain pNH 223 pLySs by Cameron (2001). Earlier work by Cameron (2001) on the production and purification of the recombinant amidase showed that the enzyme could be produced with high levels of expression in shake-flask culture. Furthermore, preliminary studies have also shown that the molecular weight of the amidase was approximately 35kDa, and that it acts optimally at a temperature and pH of 50°C and 7.2 respectively. |
| format | Thesis |
| id | oai:open.uct.ac.za:11427/6692 |
| institution | University of Cape Town (South Africa) |
| language | eng |
| last_indexed | 2026-06-10T12:34:17.944Z |
| license_str | Not specified — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UCTD — University of Cape Town Open Access Repository |
| publishDate | 2014 |
| publishDateRange | 2014 |
| publishDateSort | 2014 |
| publisher | Centre for Bioprocess Engineering Research |
| publisherStr | Centre for Bioprocess Engineering Research |
| record_format | dspace |
| source_str | UCTD — University of Cape Town Open Access Repository |
| spelling | oai:open.uct.ac.za:11427/6692 Characterization of a thermostable amidase and development of a bioreactor process for lactic acid production Makhongela, Happy Steven Burton, Stephanie Gail Cowan, DA Bioprocess Engineering This thesis reports studies of the biochemical properties of a newly isolated thermostable amidase from Bacillus sp. RAPc8, and development of a continuous reactor process for the production of a target product. The amidase was cloned and over-expressed in E. coli BL21 strain pNH 223 pLySs by Cameron (2001). Earlier work by Cameron (2001) on the production and purification of the recombinant amidase showed that the enzyme could be produced with high levels of expression in shake-flask culture. Furthermore, preliminary studies have also shown that the molecular weight of the amidase was approximately 35kDa, and that it acts optimally at a temperature and pH of 50°C and 7.2 respectively. 2014-08-28T09:15:47Z 2014-08-28T09:15:47Z 2005 Thesis http://hdl.handle.net/11427/6692 eng application/pdf Centre for Bioprocess Engineering Research Faculty of Engineering and the Built Environment University of Cape Town |
| spellingShingle | Bioprocess Engineering Makhongela, Happy Steven Characterization of a thermostable amidase and development of a bioreactor process for lactic acid production |
| title | Characterization of a thermostable amidase and development of a bioreactor process for lactic acid production |
| title_full | Characterization of a thermostable amidase and development of a bioreactor process for lactic acid production |
| title_fullStr | Characterization of a thermostable amidase and development of a bioreactor process for lactic acid production |
| title_full_unstemmed | Characterization of a thermostable amidase and development of a bioreactor process for lactic acid production |
| title_short | Characterization of a thermostable amidase and development of a bioreactor process for lactic acid production |
| title_sort | characterization of a thermostable amidase and development of a bioreactor process for lactic acid production |
| topic | Bioprocess Engineering |
| url | http://hdl.handle.net/11427/6692 |
| work_keys_str_mv | AT makhongelahappysteven characterizationofathermostableamidaseanddevelopmentofabioreactorprocessforlacticacidproduction |