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Isolation and molecular characterisation of two Pseudomonas sp. ACC deaminase genes
Bibliography: p. 125-141.
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| Main Author: | |
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| Other Authors: | |
| Format: | Thesis |
| Language: | English |
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Department of Molecular and Cell Biology
2014
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| _version_ | 1867613341679091712 |
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| access_status_str | Open Access |
| author | Campbell, Bridget Genevieve |
| author2 | Thomson, Jennifer Ann |
| author_browse | Campbell, Bridget Genevieve Thomson, Jennifer Ann |
| author_facet | Thomson, Jennifer Ann Campbell, Bridget Genevieve |
| author_sort | Campbell, Bridget Genevieve |
| collection | Thesis |
| description | Bibliography: p. 125-141. |
| format | Thesis |
| id | oai:open.uct.ac.za:11427/9650 |
| institution | University of Cape Town (South Africa) |
| language | eng |
| last_indexed | 2026-06-10T12:34:36.552Z |
| license_str | Not specified — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UCTD — University of Cape Town Open Access Repository |
| publishDate | 2014 |
| publishDateRange | 2014 |
| publishDateSort | 2014 |
| publisher | Department of Molecular and Cell Biology |
| publisherStr | Department of Molecular and Cell Biology |
| record_format | dspace |
| source_str | UCTD — University of Cape Town Open Access Repository |
| spelling | oai:open.uct.ac.za:11427/9650 Isolation and molecular characterisation of two Pseudomonas sp. ACC deaminase genes Campbell, Bridget Genevieve Thomson, Jennifer Ann Brand Reon Microbiology Bibliography: p. 125-141. The phytohormone ethylene is essential to many plant developmental processes, of which the control of climacteric fruit ripening is among the best characterised. However this hormone eventually causes fruit rotting which results in a non-marketable product. One approach to reduce ethylene synthesis in plants is metabolism of its immediate precursor, 1-aminocyclopropane-1carboxylic acid (ACC). This can be achieved through degradation of ACC by the enzyme ACC deaminase to form α-ketobutyric acid and ammonia. ACC degrading soil microorganisms were identified by their ability to grow on ACC as a sole nitrogen source. Enzyme assays indicated that Pseudomonas had high ACC deaminase gene-specific primers and probes respectively revealed that only one bacterium, Pseudomonas fluerescens strain 17, had a gene with homology to previously sequenced ACC deaminase genes. 2014-11-15T19:38:31Z 2014-11-15T19:38:31Z 1995 Master Thesis Masters MSc http://hdl.handle.net/11427/9650 eng application/pdf Department of Molecular and Cell Biology Faculty of Science University of Cape Town |
| spellingShingle | Microbiology Campbell, Bridget Genevieve Isolation and molecular characterisation of two Pseudomonas sp. ACC deaminase genes |
| thesis_degree_str | Master's |
| title | Isolation and molecular characterisation of two Pseudomonas sp. ACC deaminase genes |
| title_full | Isolation and molecular characterisation of two Pseudomonas sp. ACC deaminase genes |
| title_fullStr | Isolation and molecular characterisation of two Pseudomonas sp. ACC deaminase genes |
| title_full_unstemmed | Isolation and molecular characterisation of two Pseudomonas sp. ACC deaminase genes |
| title_short | Isolation and molecular characterisation of two Pseudomonas sp. ACC deaminase genes |
| title_sort | isolation and molecular characterisation of two pseudomonas sp acc deaminase genes |
| topic | Microbiology |
| url | http://hdl.handle.net/11427/9650 |
| work_keys_str_mv | AT campbellbridgetgenevieve isolationandmolecularcharacterisationoftwopseudomonasspaccdeaminasegenes |