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A study of the application of reduction gas analysis in determining the relationship between carboxyhaemoglobin and tissue co levels in brain, heart and skeletal muscle after extreme exposure to co poisoning in rats
Dissertation (MSc)--University of Pretoria, 2007.
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University of Pretoria
2013
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| _version_ | 1867613541462179840 |
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| access_status_str | Open Access |
| author2 | Le Roux, C.G.J. |
| author_browse | Le Roux, C.G.J. |
| author_facet | Le Roux, C.G.J. |
| collection | Thesis |
| dc_rights_str_mv | © 2006, University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Dissertation (MSc)--University of Pretoria, 2007. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/23567 |
| institution | University of Pretoria (South Africa) |
| last_indexed | 2026-06-10T12:37:45.339Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2013 |
| publishDateRange | 2013 |
| publishDateSort | 2013 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/23567 A study of the application of reduction gas analysis in determining the relationship between carboxyhaemoglobin and tissue co levels in brain, heart and skeletal muscle after extreme exposure to co poisoning in rats Le Roux, C.G.J. Vermaak, William J.H. Vreman, H.J. Piantadosi, C.A. Cronje, Frans Johannes Free radicals Reduction gas analysis Co toxicity UCTD Dissertation (MSc)--University of Pretoria, 2007. Little is known of tissue carbon monoxide (CO) changes after acute exposure because tissue levels are in the order of picomoles per milligram and the technology to measure such low concentrations has only become available relatively recently. This study tested three hypotheses: That tissue CO levels (1) vary among tissues after acute poisoning; (2) change over time; but (3) cannot be predicted by measuring carboxyhaemoglobin (COHb) levels. Twenty four healthy male Sprague-Dawley rats were exposed to 2500 ppm CO in air for 45 min. This non-lethal exposure achieved reproducible COHb values of 66 to 72%. Animals were allowed to recover breathing air and were sacrificed at 30 minute intervals for 150 minutes. An additional nine male animals served as unexposed controls. Accurate measurements of tissue CO levels were made in blood, brain, heart, and skeletal muscle samples. All samples were prepared using the validated technique described by Vreman et al., and Reduction Gas Analysis was used to determine the pmol CO per wet weight tissue. Co-oximetry and gas chromatography were performed on all blood samples. Predictably, blood CO content dropped following exposure, but tissue CO content did not follow the same trend in all tissues. This study supports the hypothesis of (1) tissue and (2) time-related variability of CO concentration in three body tissues after exposure and (3) documents lack of utility of COHb for predicting tissue CO tissue values. Nuclear Medicine Unrestricted 2013-09-06T15:36:38Z 2007-03-29 2013-09-06T15:36:38Z 2006-05-05 2007-03-29 2007-03-29 Dissertation Cronje, F 2006, A study of the application of reduction gas analysis in determining the relationship between carboxyhaemoglobin and tissue co levels in brain, heart and skeletal muscle after extreme exposure to co poisoning in rats, Magister dissertation, University of Pretoria, Pretoria, viewed yymmdd < http://hdl.handle.net/2263/23567 > http://hdl.handle.net/2263/23567 http://upetd.up.ac.za/thesis/available/etd-03292007-122020/ © 2006, University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | Free radicals Reduction gas analysis Co toxicity UCTD A study of the application of reduction gas analysis in determining the relationship between carboxyhaemoglobin and tissue co levels in brain, heart and skeletal muscle after extreme exposure to co poisoning in rats |
| title | A study of the application of reduction gas analysis in determining the relationship between carboxyhaemoglobin and tissue co levels in brain, heart and skeletal muscle after extreme exposure to co poisoning in rats |
| title_full | A study of the application of reduction gas analysis in determining the relationship between carboxyhaemoglobin and tissue co levels in brain, heart and skeletal muscle after extreme exposure to co poisoning in rats |
| title_fullStr | A study of the application of reduction gas analysis in determining the relationship between carboxyhaemoglobin and tissue co levels in brain, heart and skeletal muscle after extreme exposure to co poisoning in rats |
| title_full_unstemmed | A study of the application of reduction gas analysis in determining the relationship between carboxyhaemoglobin and tissue co levels in brain, heart and skeletal muscle after extreme exposure to co poisoning in rats |
| title_short | A study of the application of reduction gas analysis in determining the relationship between carboxyhaemoglobin and tissue co levels in brain, heart and skeletal muscle after extreme exposure to co poisoning in rats |
| title_sort | study of the application of reduction gas analysis in determining the relationship between carboxyhaemoglobin and tissue co levels in brain heart and skeletal muscle after extreme exposure to co poisoning in rats |
| topic | Free radicals Reduction gas analysis Co toxicity UCTD |
| url | http://hdl.handle.net/2263/23567 http://upetd.up.ac.za/thesis/available/etd-03292007-122020/ |