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Enzyme profiling of a range of sugarcane tissue types with different levels of sucrose
Thesisa (MSc (Genetics. Plant Biotechnology))--University of Stellenbosch, 2005.
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| Format: | Thesis |
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Stellenbosch : University of Stellenbosch
2006
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| _version_ | 1867613809625006080 |
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| access_status_str | Open Access |
| author | Orendo-Smith, R. |
| author2 | Botha, F. C. |
| author_browse | Botha, F. C. Orendo-Smith, R. |
| author_facet | Botha, F. C. Orendo-Smith, R. |
| author_sort | Orendo-Smith, R. |
| collection | Thesis |
| dc_rights_str_mv | University of Stellenbosch |
| description | Thesisa (MSc (Genetics. Plant Biotechnology))--University of Stellenbosch, 2005. |
| format | Thesis |
| id | oai:scholar.sun.ac.za:10019.1/2462 |
| institution | Stellenbosch University (South Africa) |
| last_indexed | 2026-06-10T12:42:03.173Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from SUNScholar — Stellenbosch University Repository |
| publishDate | 2006 |
| publishDateRange | 2006 |
| publishDateSort | 2006 |
| publisher | Stellenbosch : University of Stellenbosch |
| publisherStr | Stellenbosch : University of Stellenbosch |
| record_format | dspace |
| source_str | SUNScholar — Stellenbosch University Repository |
| spelling | oai:scholar.sun.ac.za:10019.1/2462 Enzyme profiling of a range of sugarcane tissue types with different levels of sucrose Orendo-Smith, R. Botha, F. C. Groenewald, J-H. Theses -- Plant biotechnology Dissertations -- Plant biotechnology Sugarcane -- Composition Sucrose -- Metabolism Enzymes Genetics Institute for Plant Biotechnology Thesisa (MSc (Genetics. Plant Biotechnology))--University of Stellenbosch, 2005. The study had two main objectives: 1) to investigate specific enzyme activity profiles at various developmental stages and to determine possible implications for sucrose metabolism, 2) to incorporate enzyme activity data of different internodes to obtain a detailed model of every stage in the tissue maturation process. The most significant findings of the regulation of sucrose accumulation in this study are centred on three main point controls in sucrose metabolism pathway. Firstly, the maturation of sugarcane internodes coincided with an increase of SPS in most genotypes, and this underlines the key role of this enzyme in sucrose accumulation. Secondly, SuSy activity (cleavage reaction) correlated negatively with sucrose concentration and hence with tissue maturation process, in most of the varieties. This finding indicates that SuSy could well be implicated in sucrose metabolism. Thirdly, in vitro PFP activity was found to be negatively correlated to sucrose content in sugarcane varieties differing in amount of sucrose. Masters 2006-11-17T07:13:02Z 2010-06-01T08:49:21Z 2006-11-17T07:13:02Z 2010-06-01T08:49:21Z 2005-12 Thesis http://hdl.handle.net/10019.1/2462 University of Stellenbosch 518854 bytes 139079 bytes application/pdf application/pdf application/pdf application/pdf Stellenbosch : University of Stellenbosch |
| spellingShingle | Theses -- Plant biotechnology Dissertations -- Plant biotechnology Sugarcane -- Composition Sucrose -- Metabolism Enzymes Genetics Institute for Plant Biotechnology Orendo-Smith, R. Enzyme profiling of a range of sugarcane tissue types with different levels of sucrose |
| title | Enzyme profiling of a range of sugarcane tissue types with different levels of sucrose |
| title_full | Enzyme profiling of a range of sugarcane tissue types with different levels of sucrose |
| title_fullStr | Enzyme profiling of a range of sugarcane tissue types with different levels of sucrose |
| title_full_unstemmed | Enzyme profiling of a range of sugarcane tissue types with different levels of sucrose |
| title_short | Enzyme profiling of a range of sugarcane tissue types with different levels of sucrose |
| title_sort | enzyme profiling of a range of sugarcane tissue types with different levels of sucrose |
| topic | Theses -- Plant biotechnology Dissertations -- Plant biotechnology Sugarcane -- Composition Sucrose -- Metabolism Enzymes Genetics Institute for Plant Biotechnology |
| url | http://hdl.handle.net/10019.1/2462 |
| work_keys_str_mv | AT orendosmithr enzymeprofilingofarangeofsugarcanetissuetypeswithdifferentlevelsofsucrose |