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Partial characterization of a bacterial acyltransferase enzyme for potential application in dairy processing
Thesis (MSc)--Stellenbosch University, 2014.
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| Format: | Thesis |
| Language: | en_ZA |
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Stellenbosch : Stellenbosch University
2014
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| _version_ | 1867613825745813504 |
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| access_status_str | Open Access |
| author | Hayward, Stefan |
| author2 | Swart, Pieter |
| author_browse | Hayward, Stefan Swart, Pieter |
| author_facet | Swart, Pieter Hayward, Stefan |
| author_sort | Hayward, Stefan |
| collection | Thesis |
| dc_rights_str_mv | Stellenbosch University |
| description | Thesis (MSc)--Stellenbosch University, 2014. |
| format | Thesis |
| id | oai:scholar.sun.ac.za:10019.1/86636 |
| institution | Stellenbosch University (South Africa) |
| language | en_ZA |
| last_indexed | 2026-06-10T12:42:17.808Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from SUNScholar — Stellenbosch University Repository |
| publishDate | 2014 |
| publishDateRange | 2014 |
| publishDateSort | 2014 |
| publisher | Stellenbosch : Stellenbosch University |
| publisherStr | Stellenbosch : Stellenbosch University |
| record_format | dspace |
| source_str | SUNScholar — Stellenbosch University Repository |
| spelling | oai:scholar.sun.ac.za:10019.1/86636 Partial characterization of a bacterial acyltransferase enzyme for potential application in dairy processing Hayward, Stefan Swart, Pieter Stellenbosch University. Faculty of Science. Dept. of Biochemistry. Milk -- Microbiology Fouling Dairy processing Dissertations -- Biochemistry Theses -- Biochemistry UCTD Thesis (MSc)--Stellenbosch University, 2014. ENGLISH ABSTRACT: This study describes: the evaluation of the current, and potential assay methods for the quantification of cholesterol, cholesteryl esters and free fatty acids in milk and the application thereof ; an account of the difficulties associated with the usage of FoodPro® Cleanline, an enzyme preparation used as processing aid, during ultra-high temperature processing of milk ; the development of activity assays which can be used for the kinetic characterization of glycerophospholipid cholesterol acyltransferase, the active enzyme in FoodPro® Cleanline ; the development of an accurate and facile activity assay, and the validation thereof, which can be used for the validation of enzyme activity prior to dosage of milk with FoodPro® Cleanline. AFRIKAANSE OPSOMMING: Hierdie studie beskryf: die evaluering van die huidige, en potensiële, metodes vir die kwantifisering van cholesterol, cholesteriel esters en vryvetsure in melk, sowel as die toepassing van hieridie metodes ; 'n verduideliking van die moeilikhede wat ondervind word gedurende die gebruik van FoodPro® Cleanline, 'n ensiempreparaat vir gebruik as 'n verwerkingshulpmiddel, tydens ultrahoë-temperatuurprosessering van melk ; die ontwikkeling van aktiwiteitsbepalings metodes vir gebruik in kinetiese karakterisering van gliserofosfolipied cholesterol asieltransferase, die aktiewe ensiem in FoodPro® Cleanline ; die ontwikkeling van 'n akkurate, eenvoudige aktiwiteitsbepalings metode, en bevestiging van hierdie metode, wat gebruik kan word vir kwalitieitskontrole alvorens die dosering van melk met FoodPro® Cleanline. 2014-04-16T17:30:49Z 2014-04-16T17:30:49Z 2014-04 Thesis http://hdl.handle.net/10019.1/86636 en_ZA Stellenbosch University xv, 118 p. : ill. application/pdf Stellenbosch : Stellenbosch University |
| spellingShingle | Milk -- Microbiology Fouling Dairy processing Dissertations -- Biochemistry Theses -- Biochemistry UCTD Hayward, Stefan Partial characterization of a bacterial acyltransferase enzyme for potential application in dairy processing |
| title | Partial characterization of a bacterial acyltransferase enzyme for potential application in dairy processing |
| title_full | Partial characterization of a bacterial acyltransferase enzyme for potential application in dairy processing |
| title_fullStr | Partial characterization of a bacterial acyltransferase enzyme for potential application in dairy processing |
| title_full_unstemmed | Partial characterization of a bacterial acyltransferase enzyme for potential application in dairy processing |
| title_short | Partial characterization of a bacterial acyltransferase enzyme for potential application in dairy processing |
| title_sort | partial characterization of a bacterial acyltransferase enzyme for potential application in dairy processing |
| topic | Milk -- Microbiology Fouling Dairy processing Dissertations -- Biochemistry Theses -- Biochemistry UCTD |
| url | http://hdl.handle.net/10019.1/86636 |
| work_keys_str_mv | AT haywardstefan partialcharacterizationofabacterialacyltransferaseenzymeforpotentialapplicationindairyprocessing |