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Construction and analysis of a kinetic model of the ovine adrenal steroidogenic subsystem comprising of CYP17 and 3β-HSD
ENGLISH ABSTRACT : This study describes: how time-dependent experimental data, describing the reactions catalyzed by ovine cytochrome P450 17α-hydroxylase/17,20-lyase (CYP17), was obtained using pregnenolone and progesterone as substrates ; the construction and validation of a kinetic model and...
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| Format: | Thesis |
| Language: | en_ZA |
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Stellenbosch : Stellenbosch University
2016
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| _version_ | 1867613785991151616 |
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| access_status_str | Open Access |
| author | Van Schalkwyk, Frederick Josephus (Erick) |
| author2 | Swart, Pieter |
| author_browse | Swart, Pieter Van Schalkwyk, Frederick Josephus (Erick) |
| author_facet | Swart, Pieter Van Schalkwyk, Frederick Josephus (Erick) |
| author_sort | Van Schalkwyk, Frederick Josephus (Erick) |
| collection | Thesis |
| dc_rights_str_mv | Stellenbosch University |
| description | ENGLISH ABSTRACT : This study describes: how time-dependent experimental data, describing the reactions catalyzed by ovine
cytochrome P450 17α-hydroxylase/17,20-lyase (CYP17), was obtained using
pregnenolone and progesterone as substrates ;
the construction and validation of a kinetic model and determining the Km and Vmax -
values for each reaction catalyzed by ovine CYP17 ; how the time-dependent metabolism of pregnenolone, 17α-hydroxypregnenolone and
dehydroepiandrosterone by ovine 3β-hydroxysteroid dehydrogenase (3β-HSD), was
obtained ;
the construction and validation of a kinetic model and determining the Km and Vmax -
values for each reaction catalyzed by ovine 3β-HSD |
| format | Thesis |
| id | oai:scholar.sun.ac.za:10019.1/98727 |
| institution | Stellenbosch University (South Africa) |
| language | en_ZA |
| last_indexed | 2026-06-10T12:41:40.401Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from SUNScholar — Stellenbosch University Repository |
| publishDate | 2016 |
| publishDateRange | 2016 |
| publishDateSort | 2016 |
| publisher | Stellenbosch : Stellenbosch University |
| publisherStr | Stellenbosch : Stellenbosch University |
| record_format | dspace |
| source_str | SUNScholar — Stellenbosch University Repository |
| spelling | oai:scholar.sun.ac.za:10019.1/98727 Construction and analysis of a kinetic model of the ovine adrenal steroidogenic subsystem comprising of CYP17 and 3β-HSD Van Schalkwyk, Frederick Josephus (Erick) Swart, Pieter Snoep, Jacob Leendert Stellenbosch University. Faculty of Science. Department of Biochemistry Steroid hormones Biosynthesis -- Models Steroidogenesis -- Models Adrenocortical hormones -- Models Kidneys -- Physiology Sheep -- Physiology UCTD ENGLISH ABSTRACT : This study describes: how time-dependent experimental data, describing the reactions catalyzed by ovine cytochrome P450 17α-hydroxylase/17,20-lyase (CYP17), was obtained using pregnenolone and progesterone as substrates ; the construction and validation of a kinetic model and determining the Km and Vmax - values for each reaction catalyzed by ovine CYP17 ; how the time-dependent metabolism of pregnenolone, 17α-hydroxypregnenolone and dehydroepiandrosterone by ovine 3β-hydroxysteroid dehydrogenase (3β-HSD), was obtained ; the construction and validation of a kinetic model and determining the Km and Vmax - values for each reaction catalyzed by ovine 3β-HSD AFRIKAANSE OPSOMMING : Hierdie studie beskryf: hoe tyd-afhanklike eksperimentele data van die reaksies wat gekataliseer word deur skaap sitochroom P450 17α-hidroksielase/17,20-liase (CYP17) met pregnenoloon en progesteroon as substraat, verkry is ; die konstruksie en validasie van die kinetiese model asook die bepaling van die Km - en Vmax - waardes vir elke reaksie wat deur skaap CYP17 gekataliseer word ; hoe die tyd-afhanklike metabolisme van pregnenoloon, progesteroon en dehidroepiandrosteroon wat gekataliseer word deur skaap 3β-hidroksiesteroïd dehidroginase (3β-HSD), verkry is ; die konstruksie en validasie van die kinetiese model asook die bepaling van die Km - en Vmax - waardes vir elke reaksie wat deur skaap 3β-HSD gekataliseer word Thesis (MSc)--Stellenbosch University, 2016 2016-03-09T14:54:15Z 2016-03-09T14:54:15Z 2016-03 Thesis http://hdl.handle.net/10019.1/98727 en_ZA Stellenbosch University xiii, 125 pages : illustrations (chiefly colour) application/pdf Stellenbosch : Stellenbosch University |
| spellingShingle | Steroid hormones Biosynthesis -- Models Steroidogenesis -- Models Adrenocortical hormones -- Models Kidneys -- Physiology Sheep -- Physiology UCTD Van Schalkwyk, Frederick Josephus (Erick) Construction and analysis of a kinetic model of the ovine adrenal steroidogenic subsystem comprising of CYP17 and 3β-HSD |
| title | Construction and analysis of a kinetic model of the ovine adrenal steroidogenic subsystem comprising of CYP17 and 3β-HSD |
| title_full | Construction and analysis of a kinetic model of the ovine adrenal steroidogenic subsystem comprising of CYP17 and 3β-HSD |
| title_fullStr | Construction and analysis of a kinetic model of the ovine adrenal steroidogenic subsystem comprising of CYP17 and 3β-HSD |
| title_full_unstemmed | Construction and analysis of a kinetic model of the ovine adrenal steroidogenic subsystem comprising of CYP17 and 3β-HSD |
| title_short | Construction and analysis of a kinetic model of the ovine adrenal steroidogenic subsystem comprising of CYP17 and 3β-HSD |
| title_sort | construction and analysis of a kinetic model of the ovine adrenal steroidogenic subsystem comprising of cyp17 and 3β hsd |
| topic | Steroid hormones Biosynthesis -- Models Steroidogenesis -- Models Adrenocortical hormones -- Models Kidneys -- Physiology Sheep -- Physiology UCTD |
| url | http://hdl.handle.net/10019.1/98727 |
| work_keys_str_mv | AT vanschalkwykfrederickjosephuserick constructionandanalysisofakineticmodeloftheovineadrenalsteroidogenicsubsystemcomprisingofcyp17and3bhsd |